Amylo-Glo RTD Amyloid Plaque Stain Reagent with EtBr counter stain

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Catalog Number
TR-400

    Product Info

  • Product Name Amylo-Glo RTD Amyloid Plaque Stain Reagent with EtBr counter stain
  • Product Description google The Biosensis AG-400-AG kit utilizes an ethidium bromide counter stain for a quick and effective way to visualize cell nuclei and cell bodies of cells while under UV illumination allowing the assessment of amyloid plaques and cell/tissue positioning as well in one step.

    Amylo-Glo RTD Ready to Dilute Staining reagent is designed to stain amyloid plaques in tissue sections. This novel marker has several advantages over other conventional markers such as Thioflavin S and Congo Red because of its unique chemical and spectral properties. (L. Schmued et al. (2012) J.Neuroscience Methods 209:120- 126). Using Amylo-Glo results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination for multiple immunofluorescent labeling studies. Amylo-Glo RTD is compatible with fresh, frozen, and formalin-fixed immunohistochemistry or cytochemistry, and it is particularly good for confocal and multiple labeling because of its high fluorescent intensity and high resistance to photo-bleaching. Moreover because Amylo-Glo fluoresces in the UV channel, double and triple labeling experiments can be performed very easily (see protocol).
  • Alternative Names AmyloGlo
  • Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded
  • Specificity Amyloid plaques both intraneuronal and vascular for A-G, Etbr, nuclei and cell bodies both DNA and RNA label
  • Species Reactivity Human, Mouse, Other Mammals (Predicted), Rat
  • Concentration 10X
  • Purity Description Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of two fluorescent isomers. No amount of starting material was detected.
  • Regulatory Status For research use only.

    Specifications

  • Product Description The Biosensis AG-400-AG kit utilizes an ethidium bromide counter stain for a quick and effective way to visualize cell nuclei and cell bodies of cells while under UV illumination allowing the assessment of amyloid plaques and cell/tissue positioning as well in one step.

    Amylo-Glo RTD Ready to Dilute Staining reagent is designed to stain amyloid plaques in tissue sections. This novel marker has several advantages over other conventional markers such as Thioflavin S and Congo Red because of its unique chemical and spectral properties. (L. Schmued et al. (2012) J.Neuroscience Methods 209:120- 126). Using Amylo-Glo results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination for multiple immunofluorescent labeling studies. Amylo-Glo RTD is compatible with fresh, frozen, and formalin-fixed immunohistochemistry or cytochemistry, and it is particularly good for confocal and multiple labeling because of its high fluorescent intensity and high resistance to photo-bleaching. Moreover because Amylo-Glo fluoresces in the UV channel, double and triple labeling experiments can be performed very easily (see protocol).
  • Related Products Amyloid beta peptide (A-beta 40/42), Mouse Monoclonal Antibody
    Amylo-Glo RTD Amyloid Plaque Stain Reagent
  • Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded
  • Application Details Staining of amyloid plaques in human and animal tissues, see included protocol. EtBr counter stain stains nuclei and cell bodies for easy identification and spacial orientation.
  • Target Amyloid plaque
  • Specificity Amyloid plaques both intraneuronal and vascular for A-G, Etbr, nuclei and cell bodies both DNA and RNA label
  • Target Host Species Species Independent
  • Species Reactivity Human, Mouse, Other Mammals (Predicted), Rat
  • Ex/Em Max Excitation Peak: 334 nm; Emission Peak: 533 nm - unbound, 438 nm when bound to amyloid. To visualize Amylo-glo in tissue, UV light is required. For example, Amylo-Glo tissue can be examined using an epifluoresent microscope with UV (Nikon UV-2A) filter cube. Excitation (325-375 nm) Emission (400-450 nm) is typical. Also note, it is not uncommon for Amylo-Glo to appear light yellow when examined by eye, yet appear a light blue color when photographed.
    Visualization of EtBr: Ethidium bromide has an excitation peak of 300 nm and an emission peak 595 nm. Most UV compatible filter sets can be used.
  • Detection Method Fluorescence
  • Kit Components 1 bottle containing 40 mL of 10X Amylo-Glo RTD (A-G RTD) solution 1 bottle containing 40 mL of 10X A-G RTD Ethidium Bromide (EtBr RTD) solution
  • Purity Description Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of two fluorescent isomers. No amount of starting material was detected.
  • Format The reagents in the Amyloid Plaque Stain Reagent (100x) are all supplied in a liquid format and are ready-to-dilute.
  • Concentration 10X
  • Reconstitution Instructions Ready to dilute per protocol 10X solutions
  • Storage Instructions The stock solution can be stored for up to 6 months at 2-8°C protected from light. No preservatives. Use sterile technique when handling and proper laboratory procedures.
  • Batch Number Please see item label.
  • Expiration Date 6 months after date of receipt (unopened vial).
  • Alternative Names AmyloGlo
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 60103920
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Combined Amylo-Glo labeling of amyloid plaques (blue)with ethidium bromide Nissl (cell body) counterstain (red) in the dentate gyrus region of the hippocampus of the AD/Tg mouse.UV light excitation.

  • Kit Protocol

    TR-400-AG_as_at_March2020.pdf

  • SDS Link

    MSDS_TR-400-AG_as_at_July2015.pdf

    Citations & References

  • Specific References Emre C et al. (2020) "Receptors for pro-resolving mediators are increased in Alzheimer's disease brain." Brain Pathol. [Epub ahead of print]; Application: IHC/IF Species: Human

    Hascup KN et al. (2019) "LY379268 Does Not Have Long-Term Procognitive Effects nor Attenuate Glutamatergic Signaling in AβPP/PS1 Mice." J Alzheimers Dis. [Epub ahead of print]; Application: IHC/IF Species: Mouse

    Hascup ER et al. (2018) "Diet-Induced Insulin Resistance Elevates Hippocampal Glutamate as well as VGLUT1 and GFAP Expression in AβPP/PS1 Mice." J Neurochem. [Epub ahead of print]; Application: IHC/IF Species: Mouse
  • General References L. Schmued et al. (2012) J.Neuroscience Methods 209:120, 126