Amylo-Glo RTD Amyloid Plaque Stain Reagent

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Catalog Number
TR-300

    Product Info

  • Product Name Amylo-Glo RTD Amyloid Plaque Stain Reagent
  • Product Description google Amylo-Glo RTD Ready to Dilute Staining reagent is designed to stain amyloid plaques in tissue sections. This novel marker has several advantages over other conventional markers such as Thioflavin S and Congo Red because of its unique chemical and spectral properties. (L. Schmued et al. (2012) J.Neuroscience Methods 209:120- 126). Using Amylo-Glo results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination for multiple immunofluorescent labeling studies. Amylo-Glo RTD is compatible with fresh, frozen, and formalin-fixed immunohistochemistry or cytochemistry, and it is particularly good for confocal and multiple labeling because of its high fluorescent intensity and high resistance to photo-bleaching. Moreover because Amylo-Glo fluoresces in the UV channel, double and triple labeling experiments can be performed very easily (see protocol).
  • Alternative Names AmyloGlo
  • Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded
  • Specificity Amyloid plaques both intraneuronal and vascular
  • Species Reactivity Human, Mouse, Other Mammals (Predicted), Rat
  • Concentration 100X
  • Purity Description Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of two fluorescent isomers. No amount of starting material was detected.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Amylo-Glo RTD Ready to Dilute Staining reagent is designed to stain amyloid plaques in tissue sections. This novel marker has several advantages over other conventional markers such as Thioflavin S and Congo Red because of its unique chemical and spectral properties. (L. Schmued et al. (2012) J.Neuroscience Methods 209:120- 126). Using Amylo-Glo results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination for multiple immunofluorescent labeling studies. Amylo-Glo RTD is compatible with fresh, frozen, and formalin-fixed immunohistochemistry or cytochemistry, and it is particularly good for confocal and multiple labeling because of its high fluorescent intensity and high resistance to photo-bleaching. Moreover because Amylo-Glo fluoresces in the UV channel, double and triple labeling experiments can be performed very easily (see protocol).
  • Related Products Amyloid beta peptide (A-beta 40/42), Mouse Monoclonal Antibody
    Amylo-Glo RTD Amyloid Plaque Stain Reagent with EtBr counter stain
  • Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded
  • Application Details Staining of amyloid plaques in human and animal tissues, see included protocol
  • Target Amyloid plaque
  • Specificity Amyloid plaques both intraneuronal and vascular
  • Target Host Species Species Independent
  • Species Reactivity Human, Mouse, Other Mammals (Predicted), Rat
  • Ex/Em Max Excitation Peak: 334 nm; Emission Peak: 533 nm - unbound, 438 nm when bound to amyloid. To visualize Amylo-glo in tissue, UV light is required. For example, Amylo-Glo tissue can be examined using an epifluoresent microscope with UV (Nikon UV-2A) filter cube. Excitation (325-375 nm) Emission (400-450 nm) is typical. Also note, it is not uncommon for Amylo-Glo to appear light yellow when examined by eye, yet appear a light blue color when photographed.
  • Detection Method Fluorescence
  • Kit Components 5 mL of 100X Amylo-Glo RTD (A-G RTD) solution
  • Purity Description Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of two fluorescent isomers. No amount of starting material was detected.
  • Format The reagents in the Amyloid Plaque Stain Reagent (100x) are all supplied in a liquid format and are ready-to-dilute.
  • Concentration 100X
  • Reconstitution Instructions Ready to dilute per protocol; 100X
  • Storage Instructions The stock solution can be stored for up to 6 months after date of receipt at 2-8°C protected from light. No preservatives. Use sterile technique when handling and proper laboratory procedures.
  • Batch Number Please see item label.
  • Expiration Date 6 months after date of receipt (unopened vial).
  • Alternative Names AmyloGlo
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 60103920
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • This triple exposure allows for the simultaneous localization of Amylo-Glo® positive amyloid plaques (blue), GFAP positive hypertrophied astrocytes (green) and activated microglia (red) in the hippocampus of the AD/Tg mouse.Combined UV, blue and green light illumination.

  • Amylo-Glo UV illumination only


    Staining of amyloid plaques (blue) in J20 line heterozygote adult mouse brain. Image represents 3-D reconstruction from confocal z-stack. Red: astrocytes stained for GFAP. Green: Aggregates of cofilin rods. Method: brain was fixed by perfusion with 4% paraformaldehyde cut at 30 μm thickness on freezing microtome, and stained as free floating sections. Picture courtesy of Dr. John Chilton, University of Exeter, www.axonology.com.

  • Kit Protocol

    TR-300-AG_as_at_March2020.pdf

  • SDS Link

    MSDS_TR-300-AG_as_at_July2015.pdf

    Citations & References

  • Specific References Tsay HJ et al. (2021) "EK100 and Antrodin C Improve Brain Amyloid Pathology in APP/PS1 Transgenic Mice by Promoting Microglial and Perivascular Clearance Pathways." Int J Mol Sci. 22(19):10413; Application: IHC/IF Species: Mouse

    Henningfield CM et al. (2021) "Microglia-specific ApoE knock-out does not alter Alzheimer's disease plaque pathogenesis or gene expression." Glia. [Epub ahead of print]; Application: IHC/IF Species: Mouse

    Da Mesquita S et al. (2021) "Meningeal lymphatics affect microglia responses and anti-Aβ immunotherapy." Nature. 593(7858):255-260; Application: IHC/IF Species: Mouse

    Lauterborn JC et al. (2021) "Increased excitatory to inhibitory synaptic ratio in parietal cortex samples from individuals with Alzheimer's disease.” Nat Commun. 12(1):2603; Application: IHC/IF Species: Human

    Kim JH et al. (2021) "Gamma subunit of complement component 8 is a neuroinflammation inhibitor." Brain. 144(2):528-552; Application: IHC/IF Species: Mouse

    Claes C et al. (2021) "Plaque-associated human microglia accumulate lipid droplets in a chimeric model of Alzheimer's disease." Mol Neurodegener. 16(1):50; Application: IHC/IF Species: Mouse

    Crapser JD. (2021) "Investigating microglial regulation of the extracellular matrix in health and neurodegenerative disease." PhD Thesis ; Application: IHC/IF Species: Human

    Baglietto-Vargas D et al. (2021) "Generation of a humanized Aβ expressing mouse demonstrating aspects of Alzheimer's disease-like pathology." Nature Communications. 2(1):2421; Application: IHC/IF Species: Mouse

    Mistrik M et al. (2021) "Microthermal-induced subcellular-targeted protein damage in cells on plasmonic nanosilver-modified surfaces evokes a two-phase HSP-p97/VCP response." Nature Communications. 12, Article Number 719; Application: ICC/IF Species: Human

    Lemoine L et al. (2020) "Regional binding of tau and amyloid PET tracers in Down syndrome autopsy brain tissue." Mol Neurodegener. 15(1):68; Application: IHC/IF Species: Human

    Hascup KN et al. (2020) "Riluzole attenuates glutamatergic tone and cognitive decline in AβPP/PS1 mice." J Neurochem. [Epub ahead of print]; Application: IHC/IF Species: Mouse

    Holloway OG et al. (2020) "Microglia Demonstrate Local Mixed Inflammation and a Defined Morphological Shift in an APP/PS1 Mouse Model. J Alzheimers Dis. 77(4):1765-81; Application: IHC/IF Species: Mouse

    McQuade A et al. (2020) "Gene expression and functional deficits underlie TREM2-knockout microglia responses in human models of Alzheimer s disease. Nat Commun. 11(1):5370; Application: IHC/IF Species: Mouse

    Hascup KN et al. (2020) "Hippocampal alterations in glutamatergic signaling during amyloid progression in AβPP/PS1 mice." Sci Rep. 10(1):14503; Application: IHC/IF Species: Mouse

    Crapser JD et al. (2020) "Microglia facilitate loss of perineuronal nets in the Alzheimer's disease brain." EBioMedicine. 58:102919; Application: IHC/IF Species: Mouse

    Abe Y et al. (2020) "Behavioral and electrophysiological evidence for a neuroprotective role of aquaporin-4 in the 5xFAD transgenic mice model." Acta Neuropathol Commun. 8(1):67; Application: IHC/IF Species: Mouse

    Zhu X et al. (2020) "Robust neuroinflammation and perivascular pathology in rTg-DI rats, a novel model of microvascular cerebral amyloid angiopathy." J Neuroinflammation. 17(1):78; Application: IHC/IF Species: Rat

    Majewski L et al. (2020) "Transgenic Mice Overexpressing Human STIM2 and ORAI1 in Neurons Exhibit Changes in Behavior and Calcium Homeostasis but Show No Signs of Neurodegeneration." Int J Mol Sci. 21(3); Application: IHC/IF Species: Mouse

    Davtyan H et al. (2019) "Testing a MultiTEP-based combination vaccine to reduce Aβ and tau pathology in Tau22/5xFAD bigenic mice."Alzheimers Res Ther. 11(1):107; Application: IHC/IF Species: Mouse

    Yeh SHH et al. (2019) "A high-sucrose diet aggravates Alzheimer's disease pathology, attenuates hypothalamic leptin signaling, and impairs food-anticipatory activity in APPswe/PS1dE9 mice."Neurbiol. Aging. [In press]; Application: IHC/IF Species: Mouse

    Bharani KL et al. (2019) "Serum Pro-Bdnf Levels Correlate With Phospho-Tau Staining In Alzheimer's Disease."Neurbiol. Aging. [In press]; Application: IHC/IF Species: Human

    Hovakimyan A et al. (2019) "A MultiTEP platform-based epitope vaccine targeting the phosphatase activating domain (PAD) of tau: therapeutic efficacy in PS19 mice."Sci Rep. 9(1):15455; Application: IHC/IF Species: Human

    Hasselmann J et al. (2019) "Development of a Chimeric Model to Study and Manipulate Human Microglia In Vivo."Neuron. [Epub ahead of print]; Application: IHC/IF Species: Mouse

    Spangenberg E et al. (2019) "Sustained microglial depletion with CSF1R inhibitor impairs parenchymal plaque development in an Alzheimer's disease model."Nat Commun. 10(1):3758 (Supplementary Figure 1); Application: IHC/IF Species: Human

    Eggers C et al. (2019) "Novel cannabis flavonoid, cannflavin A displays both a hormetic and neuroprotective profile against amyloid _-mediated neurotoxicity in PC12 cells: comparison with geranylated flavonoids, mimulone and diplacone."Biochem Pharmacol. [Epub ahead of print]; Application: IHC/IF Species: Rat

    Dominguez E (2019) "Microglial Contributions to Alzheimer's Disease Pathogenesis." PhD Thesis, UC Irvine. Application: IHC/IF Species: Mouse

    Jovic M et al. (2019) "Short-term fish oil supplementation applied in presymptomatic stage of Alzheimer's disease enhances microglial/macrophage barrier and prevents neuritic dystrophy in parietal cortex of 5xFAD mouse model."PLoS One. 14(5):e0216726; Application: IHC/IF Species: Mouse

    Collins MJ et al. (2019) "Age moderates the effects of traumatic brain injury on beta-amyloid plaque load in APP/PS1 mice."J Neurotrauma. [Epub ahead of print]; Application: IHC/IF Species: Mouse

    Shukla AK et al. (2018) "CD11a expression distinguishes infiltrating myeloid cells from plaque-associated microglia in Alzheimer's disease."Glia. [Epub ahead of print]; Application: IHC/IF Species: Mouse

    Feng X et al. (2018) "Quantitative proteomics reveals distinct composition of amyloid plaques in Alzheimer's disease."Alzheimers Dement. [In press]; Application: IHC/IF Species: Human, mouse

    Davis J et al. (2018) "A Novel Transgenic Rat Model of Robust Cerebral Microvascular Amyloid with Prominent Vasculopathy."Am J Pathol. [Epub ahead of print]; Application: IHC/IF Species: Rat

    Palombo F et al. (2017) "Detection of Aβ plaque-associated astrogliosis in Alzheimer's disease brain by spectroscopic imaging and immunohistochemistry."Analyst. [Epub ahead of print]; Application: IF Species: Mouse

    Abud EM (2017) "Generation of Human Microglia from Induced Pluripotent Stem Cells to Study Innate Immunity in Neurological Diseases."PhD Thesis. 2017; Application: IF Species: Mouse

    Abud EM et al. (2017) "iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases."Neuron. 2017; 49(2):278-93 Application: IF Species: Mouse

    Solomon IH et al. (2017) "Brain and liver pathology, amyloid deposition, and interferon responses among older HIV-positive patients in the late HAART era."BMC Infect Dis. 2017; 17(1):151 Application: IF Species: Human

    Xu F et al. (2016) "Cerebral vascular amyloid seeds drive amyloid _-protein fibril assembly with a distinct anti-parallel structure."Nat Commun. 2016; 7:13527. Application: IF Species: Mouse

    Katsouri L et al. (2016) "PPARγ-coactivator-1_ gene transfer reduces neuronal loss and amyloid-_ generation by reducing _-secretase in an Alzheimer's disease model ."Proc Natl Acad Sci USA. 2016; 113(43):12292-97. Application: IF Species: Mouse

    Esposito G et al. (2016) "Autologous transplantation of intestine-isolated glia cells improves neuropathology and restores cognitive deficits in _ amyloid-induced neurodegeneration."Sci Rep. 2016; 6: 22605. Application: IF Species: Rat

    Marsh SE et al. (2016) "The adaptive immune system restrains Alzheimer's disease pathogenesis by modulating microglial function."Proc Natl Sci USA. Feb 16. pii: 201525466. Application: IF Species: Hu Fibrillar amyloid visualization.

    Kim YH et al. (2015) "A 3D human neural cell culture system for modeling Alzheimer's disease."Nat Protoc. Jul;10(7):985-1006. Application: IF Species: Hu, Human neural stem-cell-derived three-dimensional (3D) culture system.

    Nijholt DA et al. (2015) "Pregnancy Zone Protein is Increased in the Alzheimer's Disease Brain and Associates with Senile Plaques."J Alzheimer's Disease. 46(1):227-38. Application: IF Species: Hu

    Kamphuis W et al. (2015) "GFAP and vimentin deficiency alters gene expression in astrocytes and microglia in wild-type mice and changes the transcriptional response of reactive glia in mouse model for Alzheimer's disease."Glia. Jun;63(6):1036-56. Application: IF Species: Mouse

    Choi SH et al. (2014) "A three-dimensional human neural cell culture model of Alzheimer's disease."Nature Oct 12. doi: 10.1038/nature1380. Application: IF Species: Hu, Human neural stem-cell-derived three-dimensional (3D) culture system.

    Niedowicz DM et al. (2014). "Obesity and diabetes cause cognitive dysfunction in the absence of accelerated beta-amyloid deposition in a novel murine model of mixed or vascular dementia." Acta Neuropathol Commun. 2014 Jun 10;2:64.
  • General References L. Schmued et al. (2012) J.Neuroscience Methods 209:120, 126