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Angiotensin converting enzyme 2 (ACE2), Rabbit Polyclonal Antibody

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Catalog Number

R-1937

Product Info

Product Name Angiotensin converting enzyme 2 (ACE2), Rabbit Polyclonal Antibody
Product Description google Anti-ACE2 has no cross response to ACE1. Predicted species reactivity based on immunogen sequence: Bovine: (94%) Homology: Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Alternative Names ACE2 Antibody: ACEH, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, SARS-CoV receptor, SARS-CoV-2 receptor
Application(s) ELISA, ICC, IHC-Paraffin-embedded, WB
Antibody Host Rabbit
Antibody Type Polyclonal
Specificity Anti-ACE2 has no cross response to ACE1. Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Species Reactivity Human, Mouse, Rat
Immunogen Description Anti-ACE2 antibody (3215) was raised against a peptide corresponding to 17 amino acids near the center of human ACE2.

The immunogen is located within amino acids 180 - 230 of ACE2.
Conjugate Unconjugated
Concentration 1 mg/mL
Purity Description ACE2 Antibody is affinity chromatography purified via peptide column.
Regulatory Status For research use only.

Specifications

Product Description Anti-ACE2 has no cross response to ACE1. Predicted species reactivity based on immunogen sequence: Bovine: (94%) Homology: Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Application(s) ELISA, ICC, IHC-Paraffin-embedded, WB
Application Details WB: 1-2 µg/mL; IHC: 2 µg/mL; IF: 20 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Angiotensin converting enzyme 2 (ACE2)
Specificity Anti-ACE2 has no cross response to ACE1. Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Target Host Species Human
Species Reactivity Human, Mouse, Rat
Antibody Host Rabbit
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description Anti-ACE2 antibody (3215) was raised against a peptide corresponding to 17 amino acids near the center of human ACE2.

The immunogen is located within amino acids 180 - 230 of ACE2.
Isoform Information Human ACE2 has 2 isoforms, including isoform 1 (805aa, 93kD) and isoform 2 (555aa, 64kD). Mouse ACE2 also has 2 isoforms, including isoform 1 (805aa, 92kD) and isoform 2 (353aa, 40kD). Rat ACE2 has one isoform (805aa, 93kD). 3215 can detect human, mouse and rat.
Purity Description ACE2 Antibody is affinity chromatography purified via peptide column.
Format Liquid. ACE2 Antibody is supplied in PBS containing 0.02% sodium azide. Conc.1 mg/mL
Concentration 1 mg/mL
Reconstitution Instructions Spin vial briefly before opening.
Storage Instructions ACE2 antibody can be stored at 2-8°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names ACE2 Antibody: ACEH, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, SARS-CoV receptor, SARS-CoV-2 receptor
Uniprot Number Q9BYF1
Uniprot Number/Name Q9BYF1 (ACE2_HUMAN)
Scientific Background
ACE2 Antibody: Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. Homology modeling shows 2019-nCoV has a similar receptor-binding domain structure as SARS-CoV, which suggests COVID-19 (2019-nCoV) may use ACE2 as a receptor in humans for infection. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.
Research Area Metabolism; Infectious Disease
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues
Loading: 15 μg of lysates per lane. Antibodies: ACE2, R-1937-100 (2 μg/mL), ACE2, 3217 (2 μg/mL), ACE2, 3227 (2 μg/mL) and beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 2 Western Blot Validation in Human Tissues
Loading: 15 μg of lysates per lane. Antibodies: ACE2, R-1937-100 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 3 Western Blot Validation in Mouse Tissues
Loading: 15 μg of lysates per lane. Antibodies: ACE2, R-1937-100 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 4 Western Blot Validation in Rat Skin Tissue
Loading: 15 μg of lysates per lane. Antibodies: ACE2, R-1937-100 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 5 Immunohistochemistry Validation of ACE2 in Human Kidney Tissue
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody (R-1937-100) at 2 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 6 Immunofluorescence Validation of ACE2 in Human Testis Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling ACE-2 with R-1937-100 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Figure 7 Immunofluorescence Validation of ACE2 in Human Lung Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 with R-1937-100 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Citations & References