Product DescriptionRabbit Polyclonal Antibody to 14-3-3 sigma/Stratifin (Unconjugated), suitable for ELISA, FC, ICC, WB, IHC.
Application(s)ELISA, FC, ICC, IHC-Frozen, WB
Application DetailsEnzyme-Linked Immunosorbent Assay (ELISA), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) and Flow Cytometry (2 ug per 10^6 cells). A dilution of 0.1 – 0.5 ug/mL is recommended for ELISA and WB. A dilution of 0.5 – 1 ug/mL is recommended for IC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target14-3-3 sigma/stratifin (SFN)
SpecificityHuman, Species cross-reactivity includes mouse and rat. No cross-reactivity with other proteins
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionE.coli-derived human 14-3-3 sigma recombinant protein (Position: M1-S248).
Purity DescriptionPurified rabbit IgG
FormatLyophilized from a buffer containing 4 mg trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative Names14-3-3 protein sigma; 1433 sigma; 14-3-3 sigma; Epithelial cell marker protein 1; HME1; SFN; stratifin; YWHAS SFN YWHAS stratifin 14-3-3 protein sigma|epithelial cell marker protein 1
Scientific BackgroundStratifin (SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 4-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.
Immunofluorescence: Analysis of 14-3-3 sigma/stratifin (SFN) using anti-14-3-3 sigma/stratifin antibody in paraffin-embedded secsion of A431 cells (left), and A 549 cells (right) catalogue number R-2115-100
Left: Western Blot analysis of stratifin, catalogue number R-2115-100 Right:Immunohistochemistry of stratifin using anti-14-3-3 sigma/stratifin antibody, catalogue number R-2115-100
Flow Cytometry: Analysis of U20S cells using anti-14-3-3 sigma/stratifin antibody, catalogue number R-2115-100.