Anti-14-3-3 sigma/Stratifin (SFN) Rabbit Polyclonal Antibody

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Catalog Number
R-2115

    Product Info

  • Product Name Anti-14-3-3 sigma/Stratifin (SFN) Rabbit Polyclonal Antibody
  • Product Description google Rabbit Polyclonal Antibody to 14-3-3 sigma/Stratifin (Unconjugated), suitable for ELISA, FC, ICC, WB, IHC.
  • Alternative Names 14-3-3 protein sigma; 1433 sigma; 14-3-3 sigma; Epithelial cell marker protein 1; HME1; SFN; stratifin; YWHAS SFN YWHAS stratifin 14-3-3 protein sigma|epithelial cell marker protein 1
  • Application(s) ELISA, FC, ICC, IHC-Frozen, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity Human, Species cross-reactivity includes mouse and rat. No cross-reactivity with other proteins
  • Species Reactivity Human, Mouse, Rat
  • Immunogen Description E.coli-derived human 14-3-3 sigma recombinant protein (Position: M1-S248).
  • Conjugate Unconjugated
  • Purity Description Purified rabbit IgG
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit Polyclonal Antibody to 14-3-3 sigma/Stratifin (Unconjugated), suitable for ELISA, FC, ICC, WB, IHC.
  • Application(s) ELISA, FC, ICC, IHC-Frozen, WB
  • Application Details Enzyme-Linked Immunosorbent Assay (ELISA), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) and Flow Cytometry (2 ug per 10^6 cells). A dilution of 0.1 – 0.5 ug/mL is recommended for ELISA and WB. A dilution of 0.5 – 1 ug/mL is recommended for IC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target 14-3-3 sigma/stratifin (SFN)
  • Specificity Human, Species cross-reactivity includes mouse and rat. No cross-reactivity with other proteins
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description E.coli-derived human 14-3-3 sigma recombinant protein (Position: M1-S248).
  • Purity Description Purified rabbit IgG
  • Format Lyophilized from a buffer containing 4 mg trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names 14-3-3 protein sigma; 1433 sigma; 14-3-3 sigma; Epithelial cell marker protein 1; HME1; SFN; stratifin; YWHAS SFN YWHAS stratifin 14-3-3 protein sigma|epithelial cell marker protein 1
  • Uniprot Number P31947
  • Uniprot Number/Name P31947 (P31947.1433S_HUMAN)
  • Scientific Background Stratifin (SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 4-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Immunofluorescence: Analysis of 14-3-3 sigma/stratifin (SFN) using anti-14-3-3 sigma/stratifin antibody in paraffin-embedded secsion of A431 cells (left), and A 549 cells (right) catalogue number R-2115-100


  • Left: Western Blot analysis of stratifin, catalogue number R-2115-100 Right:Immunohistochemistry of stratifin using anti-14-3-3 sigma/stratifin antibody, catalogue number R-2115-100


    Flow Cytometry: Analysis of U20S cells using anti-14-3-3 sigma/stratifin antibody, catalogue number R-2115-100.

    Citations & References