SpecificityRecognizes native and denatured forms of Green Fluorescent Protein (GFP) and its variants; Enhanced Green Fluorescent Protein (EGFP), Yellow Fluorescent Protein (YFP), Enhanced Yellow Fluorescent Protein (EYFP) and Cyan Fluorescent Protein (CFP).
Species ReactivitySpecies Independent
Immunogen DescriptionGreen Fluorescent Protein (GFP) from the jellyfish Aequorea victoria N-Terminal peptide-KLH conjugates.
Application DetailsWestern Blotting, ELISA, Dot Blot, Immunoprecipitation, Immunostaining. Suggested dilution for Western Blotting of 1:1000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetGreen fluorescent protein (GFP)
SpecificityRecognizes native and denatured forms of Green Fluorescent Protein (GFP) and its variants; Enhanced Green Fluorescent Protein (EGFP), Yellow Fluorescent Protein (YFP), Enhanced Yellow Fluorescent Protein (EYFP) and Cyan Fluorescent Protein (CFP).
Target Host SpeciesJellyfish
Species ReactivitySpecies Independent
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone NameLGB-1
ConjugateUnconjugated
Immunogen DescriptionGreen Fluorescent Protein (GFP) from the jellyfish Aequorea victoria N-Terminal peptide-KLH conjugates.
Purity DescriptionAffinity purified
FormatLiquid. PBS, pH 7.4 with 0.05% sodium azide
Storage InstructionsStable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Western Blot analysis of Mouse monoclonal anti-GFP tag M-1305-100. Lane 1 contains a negative control. Lane 2 contains GFP fusion protein positive 293 cells (10 µg lysate) with 1 µg of anti-GFP tag antibody. Lane 3 contains GFP fusion protein positive 293 cells (10 µg lysate) with 10 µg of anti-GFP tag antibody.