Product DescriptionRabbit anti-Maltose-Binding Protein Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application DetailsWestern Blotting (WB) and ELISA. Suggested starting dilutions for WB of 1:1,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThis antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins.
Target Host SpeciesBacteria
Species ReactivitySpecies Independent
Immunogen DescriptionMBP epitope tag recombinant protein.
Purity DescriptionIgG purified, and directed against MBP
FormatLiquid. PBS, pH 7.4 with 0.05% sodium azide
Storage InstructionsStable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundMaltose binding protein (MBP) is encoded by the malE gene of E.coli. MBP is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions.