Product DescriptionMouse anti-D tag Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Application(s)ICC, IHC-Frozen, WB
Application DetailsWestern Blotting (WB), Immunostaining (IS) and Immunoprecipitation (IP). Suggested dilutions: WB at 1:100-1:1,000, IS and IP at 1:100-1:1,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityMouse monoclonal anti D-tag antibody recognises over-expressed proteins containing the D-tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Target Host SpeciesSpecies Independent
Species ReactivitySpecies Independent
Antibody IsotypeIgG2b, lambda
Immunogen DescriptionA synthetic peptide (DYKDDDDK) coupled to KLH.
FormatLiquid. PBS, pH 7.4 with 0.05% sodium azide. See vial label for concentration.
Storage InstructionsStable for 1 year at -20°C from the date of receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundThe D-tag system utilises a short hydrophilic peptide (DYKDDDDK) that is fused to either the N- or C-terminus of the protein of interest. It can be used in conjunction with other tags such as the 6X His tag.
Western Blot analysis of 293 cells transfected with D-tagged vectors and with differing concentrations of the anti-D tag M-1301-100. Lane 1 contains the highest concentration (0.5 mg/mL) and Lane 4 contains the lowest (0.1 µg/mL).