Rabbit anti-D Tag Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Application(s)ICC, IHC-Frozen, WB
Application DetailsWestern Blotting (WB), Immunostaining (IS) and Immunoprecipitation (IP). Suggested starting dilution for WB of 1:1000, IS and IP at dilutions of 1:200. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityAnti D-tag antibody recognises over-expressed proteins containing the D-tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Target Host SpeciesSpecies Independent
Species ReactivitySpecies Independent
Immunogen DescriptionA synthetic peptide (DYKDDDDK) coupled to KLH.
Purity DescriptionAffinity chromatography using epitope-specific peptide.
FormatLiquid. PBS, pH 7.4 with 0.05% sodium azide
Storage InstructionsStable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundThe D-tag system utilises a short hydrophilic peptide (DYKDDDDK) that is fused to either the N- or C-terminus of the protein of interest. It can be used in conjunction with other tags such as the 6X His tag.