Application DetailsELISA, WB, IP, IF, IHC. Western Blotting at a suggested concentration of 1:500~1:1000. ELISA at a suggested concentration of 1: 2500. IP 5 µg/sample. IF 5 µg/mL. IHC 5 µg/mL. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityHis-tagged fusion proteins
Target Host SpeciesSpecies Independent
Species ReactivitySpecies Independent
Immunogen DescriptionThe antiserum was produced by immunizing rabbit with synthesized peptide containing 6 Histidine residues.
Purity DescriptionAffinity purified
FormatLiquid. PBS, pH 7.4 with 0.02% sodium azide.
Storage InstructionsStable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundThe 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.
Western Blot analysis of Rabbit polyclonal anti-HIS tag R-1312-100. Lane 1 contains control 293 cells. Lane 2 contains 293 cells transfected with His-tagged S6. Lane 3 contains 293 cells transfected with His-tagged CREB.