ATP-binding cassette sub-family B member 5 (ABCB5), Goat Polyclonal Antibody

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Catalog Number
G-1891

    Product Info

  • Product Name ATP-binding cassette sub-family B member 5 (ABCB5), Goat Polyclonal Antibody
  • Product Description google Goat anti-ATP-binding cassette sub-family B member 5 (ABCB5) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, FC.
  • Alternative Names ATP-binding cassette, sub-family B (MDR/TAP), member 5 ; ABCB5alpha; ABCB5beta; EST422562; ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5; ABCB5 P-gp;
  • Application(s) FC, WB, Pep-ELISA
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Specificity Reacts with ABCB5 from Human. This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1).
  • Species Reactivity Human
  • Immunogen Description A synthetic peptide consisting of amino acids, C-QTQHRNTSKKAQ
  • Conjugate Unconjugated
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Goat anti-ATP-binding cassette sub-family B member 5 (ABCB5) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, FC.
  • Application(s) FC, WB, Pep-ELISA
  • Application Details Peptide ELISA (1:32000), Western Blot (0.3-1 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target ATP-binding cassette sub-family B member 5 (ABCB5)
  • Specificity Reacts with ABCB5 from Human. This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1).
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide consisting of amino acids, C-QTQHRNTSKKAQ
  • Sequence QTQHRNTSKKAQ
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
  • Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names ATP-binding cassette, sub-family B (MDR/TAP), member 5 ; ABCB5alpha; ABCB5beta; EST422562; ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5; ABCB5 P-gp;
  • Uniprot Number Q2M3G0
  • Uniprot Number/Name Q2M3G0 (ABCB5_HUMAN)
  • Scientific Background Drug efflux transporter present in a number of stem cells that acts as a regulator of cellular differentiation. Able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair (Uniprot).
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • A. Western Blot: (0.3 µg/mL) staining of lysates of cell line A431 (35 ug protein in RIPA buffer). Detected by chemiluminescence. B. Flow cytometry analysis: of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

    Citations & References