Product NameATP-binding cassette sub-family B member 5 (ABCB5), Goat Polyclonal Antibody
Product DescriptiongoogleGoat anti-ATP-binding cassette sub-family B member 5 (ABCB5) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, FC.
Alternative NamesATP-binding cassette, sub-family B (MDR/TAP), member 5 ; ABCB5alpha; ABCB5beta; EST422562; ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5; ABCB5 P-gp;
Application(s)FC, WB, Pep-ELISA
Antibody HostGoat
Antibody TypePolyclonal
SpecificityReacts with ABCB5 from Human. This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1).
Species ReactivityHuman
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-QTQHRNTSKKAQ
ConjugateUnconjugated
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Product DescriptionGoat anti-ATP-binding cassette sub-family B member 5 (ABCB5) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, FC.
Application(s)FC, WB, Pep-ELISA
Application DetailsPeptide ELISA (1:32000), Western Blot (0.3-1 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetATP-binding cassette sub-family B member 5 (ABCB5)
SpecificityReacts with ABCB5 from Human. This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1).
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostGoat
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-QTQHRNTSKKAQ
SequenceQTQHRNTSKKAQ
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FormatLiquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage InstructionsUpon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesATP-binding cassette, sub-family B (MDR/TAP), member 5 ; ABCB5alpha; ABCB5beta; EST422562; ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5; ABCB5 P-gp;
Scientific BackgroundDrug efflux transporter present in a number of stem cells that acts as a regulator of cellular differentiation. Able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair (Uniprot).
A. Western Blot: (0.3 µg/mL) staining of lysates of cell line A431 (35 ug protein in RIPA buffer). Detected by chemiluminescence. B. Flow cytometry analysis: of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.