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B-cell-activating factor (BAFF), Human, ELISA assay
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Catalog Number
BEK-2188
- Product Name B-cell-activating factor (BAFF), Human, ELISA assay
- Product Description The human B-cell-activating factor (BAFF) Kit is a sandwich ELISA. The capture antibody is a polyclonal human BAFF antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known BAFF concentration are added to these wells and allowed to complex with the bound BAFF antibody. A biotinylated human monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BAFF present in the samples. The purpose of this kit is the in-vitro quantitative determination of human BAFF in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator; BLyS; B-cell-activating factor; BAFF; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1; TALL-1; CD257; TNFSF13B; BLYS; TALL1; TNFSF20; ZTNF4;
- Application(s) ELISA
- Specificity B-cell-activating factor
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A134-L285
- Range 62.5 pg/mL - 4,000 pg/mL
- Sensitivity < 2pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human B-cell-activating factor (BAFF) Kit is a sandwich ELISA. The capture antibody is a polyclonal human BAFF antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known BAFF concentration are added to these wells and allowed to complex with the bound BAFF antibody. A biotinylated human monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BAFF present in the samples. The purpose of this kit is the in-vitro quantitative determination of human BAFF in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of B-cell-activating factor (BAFF) in Culture Supernatant, Cell Lysates, Serum, Plasma (EDTA), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target B-cell-activating factor (BAFF)
- Specificity B-cell-activating factor
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A134-L285
- Range 62.5 pg/mL - 4,000 pg/mL
- Sensitivity < 2pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator; BLyS; B-cell-activating factor; BAFF; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1; TALL-1; CD257; TNFSF13B; BLYS; TALL1; TNFSF20; ZTNF4;
- Uniprot Number Q9Y275
- Uniprot Number/Name Q9Y275 (TN13B_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.