Application DetailsELISA (50-100 ng/mL), WB (0.2 to 2 µg/mL), IF (2 to 20 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetBrain-derived neurotrophic factor (BDNF)
SpecificityDetects human, mouse, rat, guinea pig BDNF. Expected to detect BDNF from other species due to sequence homology.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Other Mammals (Predicted), Rat
Immunogen DescriptionRecombinant human mature BDNF expressed in E.coli
Purity DescriptionProtein G purified mouse IgG.
FormatLyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.1% sodium azide.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water to obtain a concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsStore lyophilized antibody at -20°C to -80°C protected from moisture. After reconstitution divide antibody into useful aliquots and keep aliquots at -20°C to -80°C for a higher stability. Working aliquots can be kept at 2-8°C for up to 1 month. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesBrain-derived neurotrophic factor; Abrineurin; proBDNF
Scientific BackgroundBDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Western blot analysis of rat astrocyte culture (Lanes 1 and 2) and rat hippocampal neuron culture lysate (Lanes 3 and 4) at two different antibody concentrations (1 µg/mL and 0.2 µg/mL).Photo courtesy of Koppel and Timmusk (Tallinn Technical University, Institute of Gene Technology).