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C-C motif chemokine 20 (MIP-3 alpha), Human, ELISA assay
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Catalog Number
BEK-2065
- Product Name C-C motif chemokine 20 (MIP-3 alpha), Human, ELISA assay
- Product Description The human MIP-3 alpha (MIP-3a) Kit is a sandwich ELISA. The capture antibody is a monoclonal human MIP-3a antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MIP-3a concentration are added to these wells and allowed to complex with the bound MIP-3a antibody. A biotinylated human MIP-3a polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MIP-3a present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MIP-3a in samples such as culture supernatant, cell lysates, serum, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names C-C motif chemokine 20; Small-inducible cytokine A20; Macrophage inflammatory protein 3 alpha; Liver and activation-regulated chemokine; CCL20; LARC; MIP3A; SCYA20;
- Application(s) ELISA
- Specificity Human MIP-3 alpha
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A27-M96
- Range 7.8 pg/mL - 500 pg/mL
- Sensitivity < 1 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human MIP-3 alpha (MIP-3a) Kit is a sandwich ELISA. The capture antibody is a monoclonal human MIP-3a antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MIP-3a concentration are added to these wells and allowed to complex with the bound MIP-3a antibody. A biotinylated human MIP-3a polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MIP-3a present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MIP-3a in samples such as culture supernatant, cell lysates, serum, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of C-C motif chemokine 20 (MIP-3 alpha) in Culture Supernatant, Cell Lysates, Serum, Plasma (EDTA), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target C-C motif chemokine 20 (MIP-3 alpha)
- Specificity Human MIP-3 alpha
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A27-M96
- Range 7.8 pg/mL - 500 pg/mL
- Sensitivity < 1 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names C-C motif chemokine 20; Small-inducible cytokine A20; Macrophage inflammatory protein 3 alpha; Liver and activation-regulated chemokine; CCL20; LARC; MIP3A; SCYA20;
- Uniprot Number P78556
- Uniprot Number/Name P78556 (CCL20_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
