Product DescriptiongoogleThe human MIP-1 alpha (MIP-1a) Kit is a sandwich ELISA. The capture antibody is a monoclonal human MIP-1a antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MIP-1a concentration are added to these wells and allowed to complex with the bound MIP-1a antibody. A biotinylated human MIP-1a polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MIP-1a present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MIP-1a in samples such as culture supernatant, serum, plasma (EDTA), plasma (Heparin) and plasma (Citrate). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Alternative NamesC-C motif chemokine 3; Small-inducible cytokine A3; Macrophage inflammatory protein 1-alpha; tonsillar lymphocyte LD78 alpha protein; G0/G1 switch regulatory protein 19-1; CCL3; G0S19-1; MIP1A; SCYA3; MIP-1 alpha;
Application(s)ELISA
SpecificityHuman MIP-1 alpha
Species ReactivityHuman
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A27-A92
Product DescriptionThe human MIP-1 alpha (MIP-1a) Kit is a sandwich ELISA. The capture antibody is a monoclonal human MIP-1a antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MIP-1a concentration are added to these wells and allowed to complex with the bound MIP-1a antibody. A biotinylated human MIP-1a polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MIP-1a present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MIP-1a in samples such as culture supernatant, serum, plasma (EDTA), plasma (Heparin) and plasma (Citrate). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Application(s)ELISA
Application DetailsELISA. For the quantification of C-C motif chemokine 3 (MIP-1 alpha) in Culture Supernatant, Serum, Plasma (EDTA), Plasma (Heparin), Plasma (Citrate). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
TargetC-C motif chemokine 3 (MIP-1 alpha)
SpecificityHuman MIP-1 alpha
Target Cross-Reactivity (ELISA)There is no detectable cross-reactivity with other relevant proteins.
Target Host SpeciesHuman
Species ReactivityHuman
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A27-A92
Kit ComponentsThe ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
Storage InstructionsStore at 2-8°C
Storage Temperature2-8°C
Batch NumberPlease see item label.
Expiration DateThis kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Alternative NamesC-C motif chemokine 3; Small-inducible cytokine A3; Macrophage inflammatory protein 1-alpha; tonsillar lymphocyte LD78 alpha protein; G0/G1 switch regulatory protein 19-1; CCL3; G0S19-1; MIP1A; SCYA3; MIP-1 alpha;
Specific ReferencesSaldana L, Valles G, Bensiamar F, Mancebo FJ, Garcia-Rey E, Vilaboa N (2017) Paracrine interactions between mesenchymal stem cells and macrophages are regulated by 1,25-dihydroxyvitamin D3. Sci Rep. 7(1):14618.
Cifuentes SC, Bensiamar F, Gallardo-Moreno AM, Osswald TA, Gonzalez-Carrasco JL, Benavente R, Gonzalez-Martin ML, Garcia-Rey E, Vilaboa N, Saldana L (2015) Incorporation of Mg particles into PDLLA regulates mesenchymal stem cell and macrophage responses. J Biomed Mater Res A. doi: 10.1002/jbm.a.35625.