SpecificityHuman, reacts with human, cow, rat, mouse. Antibody is specific for calbindin and does not recognize closely related proteins parvalbumin and calretinin as determined by Western Blotting.
Species ReactivityBovine, Human, Mouse, Rat
Immunogen DescriptionFull-length recombinant human protein
Product DescriptionChicken anti-Calbindin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Application(s)IHC-Frozen, WB
Application DetailsWestern blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCalbindin
SpecificityHuman, reacts with human, cow, rat, mouse. Antibody is specific for calbindin and does not recognize closely related proteins parvalbumin and calretinin as determined by Western Blotting.
Target Host SpeciesHuman
Species ReactivityBovine, Human, Mouse, Rat
Antibody HostChicken
Antibody TypePolyclonal
Antibody IsotypeIgY
ConjugateUnconjugated
Immunogen DescriptionFull-length recombinant human protein
Purity DescriptionIgY fraction
FormatLyophilized, without preservatives.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Storage InstructionsStore lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Left and Middle: Detection of calbindin-immunoreactivity (red) in adult rat brain cortex (Left) and striatum (Middle) by Immunohistochemistry. The calbindin antibody (1:1,000) labels a subset of sparsely-distributed interneurons (calbindin-positive interneurons) in the cortex (Left), and more densely-distributed neurons in the striatum (Middle). Since neurons also express Fox3/NeuN (M-377-100, green), calbindin-positive cells appear yellow. Insets are high magnification images of boxed area of each image. Blue: DAPI nuclear stain. IHC method: 45 um sections, fixed by transcardial perfusion with 4% paraformaldehyde. Right: Western blot analysis of calbindin expression in rat brain homogenate (Lane 1). The antibody (1:5,000) detects one single band at ~30 kDa. Specificity for calbindin is shown by probing related His-tagged calcium-binding proteins (Lane 2: Parvalbumin; Lane 3: Calretinin; Lane 4: Calbindin; 0.5 ug protein per lane). One additional band at ~60 kDa (Lane 4) most likely represents calbindin protein dimer.
Left: Analysis of calbindin expression (green, 1:2,000) in rat cerebellum by Immunohistochemistry. Sections were co-stained with rabbit anti-MeCP2 (R-1810-100, red, 1:5,000). IHC Method: Transcardial perfusion with 4% PFA, post fixation for 24 hours. Sections were cut to 45 um and stained (free-floating). Calbindin, often used as a Purkinje cell marker, is prominently detected in dendrites and perikarya of these cells in the cerebellar molecular layer. The MeCP2 antibody selectively stains nuclei of neuronal cells. Right: Western blot analysis of rat cerebellum (2), pig hippocampus (3), cow cerebellum (4), parvalbumin (6), calretinin (7), His-tagged calbindin (8). Chicken anti-calbindin (green, 1:5,000) antibody detects calbindin at ~25 kDa in tissue homogenates (2-4). Recombinant human calbindin protein (8) appears at slightly higher molecular weight due to presence of His-tag. The chicken antibody does not cross-react with closely related protein parvalbumin and calretinin.
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