Calcium/calmodulin-dependent protein kinase type II subunit alpha (CAMK2A), Goat Polyclonal Antibody

Product Info

Product Name Calcium/calmodulin-dependent protein kinase type II subunit alpha (CAMK2A), Goat Polyclonal Antibody
Product Description google Goat anti-Calcium/calmodulin-dependent protein kinase type II subunit alpha (CAMK2A) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, IHC, ICC, FC.
Alternative Names CAMK2A; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; CAMKA; KIAA0968; CaM kinase II alpha subunit; CaM-kinase II alpha chain; CaMK-II alpha subunit; OTTHUMP00000165787; OTTHUMP00000165788; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase IIA; calcium/calmodulin-dependent protein kinase type II alpha chain
Application(s) FC, ICC, IHC, WB, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with CAMK2A from Human, Mouse, Rat. This antibody is expected to recognize both reported isoforms (NP_057065.2; NP_741960.1).
Species Reactivity Human, Mouse, Rat
Immunogen Description A synthetic peptide consisting of amino acids, C-PRTAQSEETRVWHR
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Calcium/calmodulin-dependent protein kinase type II subunit alpha (CAMK2A) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, IHC, ICC, FC.
Application(s) FC, ICC, IHC, WB, Pep-ELISA
Application Details Peptide ELISA (1:164000), Western Blot (0.03-0.3 µg/mL), Immunohistochemistry (3.75 µg/mL), Immunocytochemistry (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Calcium/calmodulin-dependent protein kinase type II subunit alpha (CAMK2A)
Specificity Reacts with CAMK2A from Human, Mouse, Rat. This antibody is expected to recognize both reported isoforms (NP_057065.2; NP_741960.1).
Target Host Species Human
Species Reactivity Human, Mouse, Rat
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, C-PRTAQSEETRVWHR
Sequence PRTAQSEETRVWHR
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names CAMK2A; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; CAMKA; KIAA0968; CaM kinase II alpha subunit; CaM-kinase II alpha chain; CaMK-II alpha subunit; OTTHUMP00000165787; OTTHUMP00000165788; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase IIA; calcium/calmodulin-dependent protein kinase type II alpha chain
Uniprot Number Q9UQM7
Uniprot Number/Name Q9UQM7 (KCC2A_HUMAN)
Scientific Background Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed:28130356). Also regulates the migration of developing neurons (PubMed:29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed:23805378).
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Western Blot:(0.3 µg/mL) staining of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence. B. Flow cytometry analysis: of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
A. Human Cortex Staining: (3.8 µg/mL) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. B. Human Adrenal Gland Staining: (3.75 µg/mL) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

A. Immunofluorescence analysis: of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Immunofluorescence analysis: of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Citations & References