SpecificityHuman, reacts with human, cow, rat, mouse. Antibody is specific for calretinin and does not recognize closely related proteins parvalbumin and calbindin as determined by Western Blotting.
Species ReactivityBovine, Human, Mouse, Rat
Immunogen DescriptionFull-length recombinant human protein
Application DetailsWestern blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCalretinin (CR)
SpecificityHuman, reacts with human, cow, rat, mouse. Antibody is specific for calretinin and does not recognize closely related proteins parvalbumin and calbindin as determined by Western Blotting.
Target Host SpeciesHuman
Species ReactivityBovine, Human, Mouse, Rat
Antibody HostChicken
Antibody TypePolyclonal
Antibody IsotypeIgY
ConjugateUnconjugated
Immunogen DescriptionFull-length recombinant human protein
Purity DescriptionIgY fraction
FormatLyophilized, without preservatives.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Storage InstructionsStore lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
A: Adult rat brain hippocampus section stained for calretinin (red) and MeCP2 (green) by Immunohistochemistry. The chicken anti-Calretinin antibody labels a subset of hippocampal interneurons, which also express MeCP2 (R-1810-100). Co-staining is demonstrated in the nucleus by a yellow color. B: Adult rat cortex section co-stained for calretinin (red) and calbindin (M-1797-100, green). Each antibody specifically labels a subset of interneurons (i.e., calretinin-positive or calbindin-postive) that express each marker exclusively. Insets are high-magnification images of the boxed area in each picture. Blue: Nuclear DNA stained with DAPI. IHC method: 45 um sections; tissue was fixed by transcardial perfusion with 4% paraformaldehyde. C: Western blot of proteins parvalbumin (Lane 1), calretinin (Lane 2), calbindin (Lane 3) and rat brain homogenate (Lane 4) with chicken anti-calretinin antibody (1:1,000). Absence of immunreactivity with parvalbumin (Lane 1) and calbindin (Lane 3) demonstrates specificity of this antibody for calretinin (Lane 2). In rat brain, this antibody recognizes a single band at ~32 kDa, corresponding to calretinin.
Left: Staining of rat hippocampus with chicken anti-calretinin (green, 1:1,000) and mouse anti-parvalbumin (M-1813-100, red, 1:1,000) by Immunohistochemistry. IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The calretinin and parvalbumin antibodies label different classes of interneurons. Blue: Nuclear DNA stain. Right: Western blot analysis of calretinin expression (green, 1:1,000) in rat brain (2) and spinal cord (3), mouse brain (4) and spinal cord (5), and cow spinal cord (6). A band at 29 kDa corresponds to calretinin protein. The same blot was simultaneously probed with a mouse anti-internexin antibody (red).
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