Product DescriptionChicken anti-Casein kinase I isoform alpha (CKI-alpha) Polyclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Application(s)ICC, IHC-Frozen, WB
Application DetailsWestern Blotting (WB): 1:5,000 - 1:10,000. Casein kinase 1 alpha has a predicted molecular weight of 38 kDa.
Immunocytochemistry (ICC): 1:500-1:1,000.
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCasein kinase I isoform alpha (CKI-alpha)
SpecificityAntibody recognizes CK1 by western blot and immunohistochemistry. Cross reactivity
Target Host SpeciesHuman
Species ReactivityBovine, Chicken, Horse, Human, Mouse, Pig, Rat
Antibody HostChicken
Antibody TypePolyclonal
Antibody IsotypeIgY
ConjugateUnconjugated
Immunogen DescriptionShortest isoform of recombinant full length CK1a.
Purity DescriptionConcentrated purified IgY
FormatLyophilized, without preservatives.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration. (Reference: uniprot.org)
A: Immunofluorescence analysis of casein kinase 1 alpha expression (red) in HeLa cells with C-1371-100. Casein kinase 1 alpha immunoreactivity is observed in the nucleus and the cytoplasm. Nuclear pore complexes (green) were stained with monoclonal antibody M-1397-250. Cell nuclei were visualized with Hoechst dye (blue). B: Western blot of whole rat spinal cord homogenate. C-1371-100 (1:10,000 dilution) detects 2 bands at apparent molecular weights of ~38 kDa and ~34 kDa, corresponding to CK1 alpha and related isotypes.