Image of a rat brain stem section by Immunofluorescence and Immunohistochemistry. The section was stained with M-377-50, Neuronal nuclei antigen [NeuN] /RNA binding protein fox-1 homolog 3 [FOX3], (NeuN/FOX3), Clone 1B7, Mouse mAb, (green), and co-stained with product C-1382-50, Microtubule associated protein (MAP2), Chicken pAb, (red), and DAPI staining of nuclear DNA (blue). Product M-377-100 selectively stains nuclei and the proximal cytoplasm of neuronal cells, while product C-1382-50 labels dendrites and overlaps with Fox3/NeuN staining in the perikarya of neurons. Method: following transcardial perfusion with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45 um sections, and free-floating sections were stained with the two antibodies.