CD44, Rat, ELISA assay

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Catalog Number
BEK-2359

    Product Info

  • Product Name CD44, Rat, ELISA assay
  • Product Description google The Rat CD44 ELISA kit is a sandwich ELISA. The capture antibody is a mouse monoclonal CD44 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known CD44 concentration are added to these wells and allowed to complex with the bound CD44 antibody. A biotinylated CD44 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD44 present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat CD44 in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Alternative Names CD44 antigen; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; PGP-1; PGP-I;
  • Application(s) ELISA
  • Specificity Rat CD44
  • Species Reactivity Rat
  • Immunogen Description The standard for this kit is recombinant rat CD44 produced in NS0 cells; Immunogen sequence: Q22-E271
  • Range 312 pg/mL - 20,000 pg/mL
  • Sensitivity < 10 pg/mL
  • Regulatory Status For research use only.

    Specifications

  • Product Description The Rat CD44 ELISA kit is a sandwich ELISA. The capture antibody is a mouse monoclonal CD44 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known CD44 concentration are added to these wells and allowed to complex with the bound CD44 antibody. A biotinylated CD44 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD44 present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat CD44 in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Application(s) ELISA
  • Application Details ELISA. For the quantification of CD44 in Culture Supernatant, Cell Lysates, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
  • Target CD44
  • Specificity Rat CD44
  • Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
  • Target Host Species Rat
  • Species Reactivity Rat
  • Immunogen Description The standard for this kit is recombinant rat CD44 produced in NS0 cells; Immunogen sequence: Q22-E271
  • Range 312 pg/mL - 20,000 pg/mL
  • Sensitivity < 10 pg/mL
  • Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
  • Detection Method Colorimetric
  • Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), rat CD44 protein standards, rat CD44 biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
  • Storage Instructions Store at 2-8°C
  • Storage Temperature 2-8°C
  • Batch Number Please see item label.
  • Expiration Date 6 months after date of receipt if unopened
  • Alternative Names CD44 antigen; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; PGP-1; PGP-I;
  • Uniprot Number P26051
  • Uniprot Number/Name P26051 (CD44_RAT)
  • Scientific Background CD44 protein is also known as the receptor for hyaluronic acid (HA). CD44 mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, CD44 may play an important role in invadopodia formation, and it also is involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction of CD44 causes numerous pathogenic phenotypes. CD44 has great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116158
  • Regulatory Status For research use only.

    Citations & References