1800 605-5127
+61 (0)8 8352 7711
Cerebral dopamine neurotrophic factor (CDNF), Human, ELISA assay
As low as
US$547.00
Only %1 left
Catalog Number
BEK-2355
- Product Name Cerebral dopamine neurotrophic factor (CDNF), Human, ELISA assay
- Product Description The human CDNF kit is a sandwich ELISA. The capture antibody is a mouse monoclonal antibody to human CDNF pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known human CDNF concentration are added to these wells and allowed to complex with the bound antibody to human CDNF. A biotinylated polyclonal goat antibody to human CDNF is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of human CDNF present in the samples. The purpose of this kit is the in-vitro quantitative determination of human CDNF in samples such as culture supernatant, serum, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Cerebral dopamine neurotrophic factor; ARMET-like protein 1; Conserved dopamine neurotrophic factor; Armetl1;
- Application(s) ELISA
- Specificity Human CDNF protein
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant human CDNF expressed in an E.coli expression system; Immunogen sequence: Q25-L187
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human CDNF kit is a sandwich ELISA. The capture antibody is a mouse monoclonal antibody to human CDNF pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known human CDNF concentration are added to these wells and allowed to complex with the bound antibody to human CDNF. A biotinylated polyclonal goat antibody to human CDNF is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of human CDNF present in the samples. The purpose of this kit is the in-vitro quantitative determination of human CDNF in samples such as culture supernatant, serum, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Cerebral dopamine neurotrophic factor (CDNF) in Culture Supernatant, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Cerebral dopamine neurotrophic factor (CDNF)
- Specificity Human CDNF protein
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant human CDNF expressed in an E.coli expression system; Immunogen sequence: Q25-L187
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), human CDNF protein standards, human CDNF biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 6 months after date of receipt if unopened
- Alternative Names Cerebral dopamine neurotrophic factor; ARMET-like protein 1; Conserved dopamine neurotrophic factor; Armetl1;
- Uniprot Number Q49AH0
- Uniprot Number/Name Q49AH0 (CDNF_HUMAN)
- Scientific Background CDNF is a trophic factor for dopamine neurons. It prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
