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Cerebral dopamine neurotrophic factor (CDNF), Human, Mouse, Rat, ELISA assay
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US$547.00
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Catalog Number
BEK-2356
- Product Name Cerebral dopamine neurotrophic factor (CDNF), Human, Mouse, Rat, ELISA assay
- Product Description The Mouse CDNF ELISA kit is a sandwich ELISA. The capture antibody is a rat monoclonal CDNF antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known CDNF concentration are added to these wells and allowed to complex with the bound CDNF antibody. A biotinylated CDNF goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CDNF present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse CDNF in samples such as culture supernatant, serum, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Cerebral dopamine neurotrophic factor; ARMET-like protein 1; Conserved dopamine neurotrophic factor; Armetl1;
- Application(s) ELISA
- Specificity Mouse CDNF
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen for this kit is recombinant mouse CDNF produced in E.coli cells; Immunogen sequence: Q25-L187
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The Mouse CDNF ELISA kit is a sandwich ELISA. The capture antibody is a rat monoclonal CDNF antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known CDNF concentration are added to these wells and allowed to complex with the bound CDNF antibody. A biotinylated CDNF goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CDNF present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse CDNF in samples such as culture supernatant, serum, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Cerebral dopamine neurotrophic factor (CDNF) in Culture Supernatant, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Cerebral dopamine neurotrophic factor (CDNF)
- Specificity Mouse CDNF
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Mouse
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen for this kit is recombinant mouse CDNF produced in E.coli cells; Immunogen sequence: Q25-L187
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), mouse CDNF protein standards, mouse CDNF biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 6 months after date of receipt if unopened
- Alternative Names Cerebral dopamine neurotrophic factor; ARMET-like protein 1; Conserved dopamine neurotrophic factor; Armetl1;
- Uniprot Number Q8CC36
- Uniprot Number/Name Q8CC36 (CDNF_MOUSE)
- Scientific Background CDNF (conserved dopamine neurotrophic factor) is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson's disease. When administered after 6-OHDA or MPTP -lesioning, it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
