E3 ubiquitin-protein ligase HECW2, Goat Polyclonal Antibody

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Catalog Number
G-1930

    Product Info

  • Product Name E3 ubiquitin-protein ligase HECW2, Goat Polyclonal Antibody
  • Product Description google Goat anti-E3 ubiquitin-protein ligase HECW2 Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, FC.
  • Alternative Names HECW2 HECT C2 and WW domain containing E3 ubiquitin protein ligase 2; NEDD4-related E3 ubiquitin ligase; NEDL2; KIAA1301 protein; EndoNEDD4; DKFZp686M17164; NEDL2;
  • Application(s) FC, Pep-ELISA
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Specificity Reacts with EndoNEDD4 / NEDL2 from Human.
  • Species Reactivity Bovine (Predicted), Human, Mouse (Predicted), Rat (Predicted)
  • Immunogen Description A synthetic peptide consisting of amino acids, ASSAREHLLFVRR-C
  • Conjugate Unconjugated
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Goat anti-E3 ubiquitin-protein ligase HECW2 Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, FC.
  • Application(s) FC, Pep-ELISA
  • Application Details Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target E3 ubiquitin-protein ligase HECW2
  • Specificity Reacts with EndoNEDD4 / NEDL2 from Human.
  • Target Host Species Human
  • Species Reactivity Bovine (Predicted), Human, Mouse (Predicted), Rat (Predicted)
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide consisting of amino acids, ASSAREHLLFVRR-C
  • Sequence ASSAREHLLFVRR
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
  • Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names HECW2 HECT C2 and WW domain containing E3 ubiquitin protein ligase 2; NEDD4-related E3 ubiquitin ligase; NEDL2; KIAA1301 protein; EndoNEDD4; DKFZp686M17164; NEDL2;
  • Uniprot Number Q9P2P5
  • Uniprot Number/Name Q9P2P5 (HECW2_HUMAN)
  • Scientific Background E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73 (PubMed:12890487). Involved in the regulation of mitotic metaphase/anaphase transition(Uniprot).
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Flow cytometric analysis: paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

    Citations & References