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Emmprin (CD147), Human, ELISA assay
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Catalog Number
BEK-2175
- Product Name Emmprin (CD147), Human, ELISA assay
- Product Description The human Emmprin Kit is a sandwich ELISA. The capture antibody is a polyclonal human Emmprin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Emmprin concentration are added to these wells and allowed to complex with the bound Emmprin antibody. A biotinylated human Emmprin monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Emmprin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Emmprin in samples such as culture supernatant, serum, plasma (EDTA), plasma (Heparin), saliva, urine and human milk. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Basigin; 5F7; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; EMMPRIN; Leukocyte activation antigen M6; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor; TCSF; CD147; BSG;
- Application(s) ELISA
- Specificity Human Emmprin/CD147
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids T25-H205
- Range 31.2 pg/mL - 2,000 pg/mL
- Sensitivity < 2 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human Emmprin Kit is a sandwich ELISA. The capture antibody is a polyclonal human Emmprin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Emmprin concentration are added to these wells and allowed to complex with the bound Emmprin antibody. A biotinylated human Emmprin monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Emmprin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Emmprin in samples such as culture supernatant, serum, plasma (EDTA), plasma (Heparin), saliva, urine and human milk. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Emmprin (CD147) in Culture Supernatant, Serum, Plasma (EDTA), Plasma (Heparin), Urine, Saliva, Human Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Emmprin (CD147)
- Specificity Human Emmprin/CD147
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids T25-H205
- Range 31.2 pg/mL - 2,000 pg/mL
- Sensitivity < 2 pg/mL
- Sample Type Culture Supernatant, Human Milk, Plasma (EDTA), Plasma (Heparin), Saliva, Serum, Urine
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Basigin; 5F7; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; EMMPRIN; Leukocyte activation antigen M6; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor; TCSF; CD147; BSG;
- Uniprot Number P35613
- Uniprot Number/Name P35613 (BASI_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
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Specific References
Lu Q et al (2013) Expression and clinical significance of extracellular matrix metalloproteinase inducer, EMMPRIN/CD147, in human osteosarcoma. Oncol Lett. 2013 Jan;5(1):201-207