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Endothelin-1, Human, ELISA assay
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Catalog Number
BEK-2334
- Product Name Endothelin-1, Human, ELISA assay
-
Product Description
For quantitative detection of human Endothelin in cell culture supernatants, serum, and heparin, or EDTA treated plasma samples only when used as directed
The human Endothelin Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse Endothelin antibody pre-coated onto the 96-well strip plates provided in the kit. Standards (expression system for standard: human peptide; Immunogen sequence: C53-W73) and test samples are added to the wells. A biotinylated detection polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Endothelin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Endothelin in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Range: 3.9 pg/mL - 250 pg/mL; Sensitivity <0.5 pg/mL - Alternative Names Preproendothelin-1; PPET1; EDN1; ET-1;
- Application(s) ELISA
- Specificity Natural and recombinant Human Endothelin There is no detectable cross-reactivity with other relevant proteins.
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A33-E407
- Range 3.9 pg/mL - 250 pg/mL
- Sensitivity < 0.5 pg/mL
- Regulatory Status For research use only.
Product Info
-
Product Description
For quantitative detection of human Endothelin in cell culture supernatants, serum, and heparin, or EDTA treated plasma samples only when used as directed
The human Endothelin Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse Endothelin antibody pre-coated onto the 96-well strip plates provided in the kit. Standards (expression system for standard: human peptide; Immunogen sequence: C53-W73) and test samples are added to the wells. A biotinylated detection polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Endothelin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Endothelin in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Range: 3.9 pg/mL - 250 pg/mL; Sensitivity <0.5 pg/mL - Application(s) ELISA
- Application Details ELISA. For the quantification of Endothelin-1 in Culture Supernatant, Serum, Cell Lysates, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Endothelin-1
- Specificity Natural and recombinant Human Endothelin There is no detectable cross-reactivity with other relevant proteins.
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A33-E407
- Range 3.9 pg/mL - 250 pg/mL
- Sensitivity < 0.5 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date Store at 4°C for 6 months, at -20°C for 12 months.
- Alternative Names Preproendothelin-1; PPET1; EDN1; ET-1;
- Uniprot Number P05305
- Uniprot Number/Name P05305 (EDN1_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
