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Fibroblast growth factor 1 (FGF-1), Human, ELISA assay
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US$547.00
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Catalog Number
BEK-2341
- Product Name Fibroblast growth factor 1 (FGF-1), Human, ELISA assay
- Product Description The Human FGF1 ELISA is a solid phase immunoassay specifically designed to measure Human FGF1. The capture antibody is a mouse monoclonal FGF1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known FGF1 concentration are added to these wells and allowed to complex with the bound FGF1 antibody. A biotinylated FGF1 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of FGF1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human FGF1 in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures
- Alternative Names Fibroblast growth factor 1; Acidic fibroblast growth factor; Endothelial cell growth factor; Heparin-binding growth factor 1; FGF-1; aFGF; ECGF; HBGF-1;
- Application(s) ELISA
- Specificity Human FGF1
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant Human FGF1 produced in E.coli cells; Immunogen sequence: A2-D155.
- Range 31.2 pg/mL - 2,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The Human FGF1 ELISA is a solid phase immunoassay specifically designed to measure Human FGF1. The capture antibody is a mouse monoclonal FGF1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known FGF1 concentration are added to these wells and allowed to complex with the bound FGF1 antibody. A biotinylated FGF1 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of FGF1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human FGF1 in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures
- Application(s) ELISA
- Application Details ELISA. For the quantification of Fibroblast growth factor 1 (FGF-1) in Culture Supernatant, Cell Lysates, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Fibroblast growth factor 1 (FGF-1)
- Specificity Human FGF1
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant Human FGF1 produced in E.coli cells; Immunogen sequence: A2-D155.
- Range 31.2 pg/mL - 2,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), human FGF1 protein standards, human FGF1 biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample diluent, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 6 months after date of receipt if unopened
- Alternative Names Fibroblast growth factor 1; Acidic fibroblast growth factor; Endothelial cell growth factor; Heparin-binding growth factor 1; FGF-1; aFGF; ECGF; HBGF-1;
- Uniprot Number P05230
- Uniprot Number/Name P05230 (FGF1_HUMAN)
- Scientific Background FGF1 is important in cell survival, cell division, angigenesis, cell differentiation and cell migration.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
