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Fibroblast growth factor 2 (FGF2), Human, ELISA assay
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US$547.00
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Catalog Number
BEK-2363
- Product Name Fibroblast growth factor 2 (FGF2), Human, ELISA assay
- Product Description The human fibroblast growth factor 2 (FGF2) Kit is a sandwich ELISA. The capture antibody is a human FGF2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known FGF2 concentration are added to these wells and allowed to complex with the bound FGF2 antibody. A biotinylated human FGF2 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of FGF2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human FGF2 in samples such as culture supernatant, serum, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2;
- Application(s) ELISA
- Specificity Human FGF-2, there is no detectable cross-reactivity with other relevant proteins.
- Species Reactivity Human
- Immunogen Description Standard and antigen for this kit is recombinant human FGF-2, amino acids 143-288, expressed in and purified from E.coli.
- Range 62.5 - 4,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human fibroblast growth factor 2 (FGF2) Kit is a sandwich ELISA. The capture antibody is a human FGF2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known FGF2 concentration are added to these wells and allowed to complex with the bound FGF2 antibody. A biotinylated human FGF2 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of FGF2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human FGF2 in samples such as culture supernatant, serum, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Fibroblast growth factor 2 (FGF2) in Culture Supernatant, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Fibroblast growth factor 2 (FGF2)
- Specificity Human FGF-2, there is no detectable cross-reactivity with other relevant proteins.
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description Standard and antigen for this kit is recombinant human FGF-2, amino acids 143-288, expressed in and purified from E.coli.
- Range 62.5 - 4,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plates(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 6 months after date of receipt if unopened
- Alternative Names Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2;
- Uniprot Number P09038
- Uniprot Number/Name P09038 (FGF2_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
