Product DescriptiongoogleThe rat Fibronectin Kit is a sandwich ELISA. The capture antibody is a monoclonal rat Fibronectin antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known Fibronectin concentration are added to these wells and allowed to complex with the bound Fibronectin antibody. A biotinylated anti-rat Fibronectin antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Fibronectin present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat Fibronectin in samples such as culture supernatant, serum, plasma (EDTA), plasma (Citrate) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Alternative NamesFN; Cold-insoluble globulin; CIG; Anastellin; FN1;
Application(s)ELISA
SpecificityRat Fibronectin
Species ReactivityRat
Immunogen DescriptionPurified Rat Fibronectin from plasma
Product DescriptionThe rat Fibronectin Kit is a sandwich ELISA. The capture antibody is a monoclonal rat Fibronectin antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known Fibronectin concentration are added to these wells and allowed to complex with the bound Fibronectin antibody. A biotinylated anti-rat Fibronectin antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Fibronectin present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat Fibronectin in samples such as culture supernatant, serum, plasma (EDTA), plasma (Citrate) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Application(s)ELISA
Application DetailsELISA. For the quantification of Fibronectin (FN) in Culture Supernatant, Serum, Plasma (EDTA), Plasma (Citrate), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
TargetFibronectin (FN)
SpecificityRat Fibronectin
Target Cross-Reactivity (ELISA)There is no detectable cross-reactivity with other relevant proteins.
Target Host SpeciesRat
Species ReactivityRat
Immunogen DescriptionPurified Rat Fibronectin from plasma
Kit ComponentsThe ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
Storage InstructionsStore at 2-8°C
Storage Temperature2-8°C
Batch NumberPlease see item label.
Expiration DateThis kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Alternative NamesFN; Cold-insoluble globulin; CIG; Anastellin; FN1;