Product NameGATA-type zinc finger protein 1 (ZGLP1), Goat Polyclonal Antibody
Product DescriptiongoogleGoat anti-GATA-type zinc finger protein 1 (ZGLP1) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Alternative NamesZGLP1; zinc finger, GATA-like protein 1; FLJ39511; FLJ39703; GLP-1; GLP1; GATA like protein 1; GATA like protein-1
Application(s)FC, ICC, Pep-ELISA
Antibody HostGoat
Antibody TypePolyclonal
SpecificityReacts with ZGLP1 from Human.
Species ReactivityBovine, Human, Mouse
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-EGVTLKFQIKPDS
ConjugateUnconjugated
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Product DescriptionGoat anti-GATA-type zinc finger protein 1 (ZGLP1) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Application(s)FC, ICC, Pep-ELISA
Application DetailsPeptide ELISA (1:16000), Western Blot (0.1-1 µg/mL), Immunocytochemistry (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetGATA-type zinc finger protein 1 (ZGLP1)
SpecificityReacts with ZGLP1 from Human.
Target Host SpeciesHuman
Species ReactivityBovine, Human, Mouse
Antibody HostGoat
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-EGVTLKFQIKPDS
SequenceEGVTLKFQIKPDS
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FormatLiquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage InstructionsUpon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesZGLP1; zinc finger, GATA-like protein 1; FLJ39511; FLJ39703; GLP-1; GLP1; GATA like protein 1; GATA like protein-1
Scientific BackgroundTranscriptional repressor that plays a central role in somatic cells of the gonad and is required for germ cell development. Able to repress GATA transcription factor function
A. Immunofluorescenceanalysis: of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and vesicle staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Immunofluorescenceanalysis: of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Flow cytometric analysis: of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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