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Ghrelin, Guinea pig Polyclonal Antibody
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Catalog Number
GP-032
- Product Name Ghrelin, Guinea pig Polyclonal Antibody
- Product Description Guinea Pig anti-Ghrelin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
- Alternative Names Appetite-regulating hormone; Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; M46 protein
- Application(s) IHC-Frozen
- Antibody Host Guinea Pig
- Antibody Type Polyclonal
- Specificity Specificity was demonstrated by immunohistochemistry. This antibody is known to react with sheep and human. Other species have not yet been tested.
- Species Reactivity Human, Sheep
- Immunogen Description A synthetic peptide (GSSFLSPEHQRVQQRKESKKPPAKLQPR) corresponding to the amino acids 24-51 from human ghrelin. This peptide was conjugated to carrier protein to enhance the immunological response.
- Conjugate Unconjugated
- Purity Description Neat serum.
- Regulatory Status For research use only.
Product Info
- Product Description Guinea Pig anti-Ghrelin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
- Application(s) IHC-Frozen
- Application Details IHC. A concentration of 1 in 3000 is recommended for IHC. IHC performed in sheep abomasum demonstrates intense staining of cells and terminals. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/mL of human ghrelin. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Ghrelin
- Specificity Specificity was demonstrated by immunohistochemistry. This antibody is known to react with sheep and human. Other species have not yet been tested.
- Target Host Species Human
- Species Reactivity Human, Sheep
- Antibody Host Guinea Pig
- Antibody Type Polyclonal
- Antibody Isotype Mixed
- Conjugate Unconjugated
- Immunogen Description A synthetic peptide (GSSFLSPEHQRVQQRKESKKPPAKLQPR) corresponding to the amino acids 24-51 from human ghrelin. This peptide was conjugated to carrier protein to enhance the immunological response.
- Purity Description Neat serum.
- Format Lyophilized
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
- Storage Instructions It is recommended that a thawed sample is stored at 2-8°C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20°C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Appetite-regulating hormone; Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; M46 protein
- Uniprot Number Q9UBU3
- Uniprot Number/Name Q9UBU3 (GHRL_HUMAN)
- Scientific Background Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) and upon binding to the receptor it induces the release of growth hormone from the pituitary. This ligand has an appetite-stimulating effect and is involved in growth regulation.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
Specifications
- General References Kojima M, Hosoda H, Matsuo H, Kangawa K (2001). Ghrelin; discovery of the natural endogenous ligand for the growth hormone secretagogue receptor. Trends Endocrinol Metab 12 pp 118-122.