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Glial cell line-derived neurotrophic factor (GDNF), Human, Rapid™ ELISA assay
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Catalog Number
BEK-2222
- Product Name Glial cell line-derived neurotrophic factor (GDNF), Human, Rapid™ ELISA assay
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Product Description
The Biosensis GDNF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and guinea pig serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.
This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards.
This GDNF ELISA kit employs a recombinant human GDNF standard obtained from the National Institute for Biological Standards and Control (NIBSC) and is therefore designed to accurately measure the human form of GDNF. Note that the antibodies used in this kit cross-react with guinea pig, rat and mouse GDNF.
Guinea pig GDNF has been quantified in serum using the Human GDNF RapidTM ELISA kit, since guinea pig GDNF protein shares closest amino acid sequence homology with human GDNF. Protein levels can be expressed as human GDNF equivalents.
This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures. - Alternative Names Glial cell line-derived neurotrophic factor; Astrocyte-derived trophic factor; ATF; GDNF;
- Application(s) ELISA
- Specificity Human. The antibodies used in this kit detect guinea pig, mouse and rat GDNF. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
- Species Reactivity Human
- Immunogen Description Human recombinant GDNF with N-terminal methionine residue.
- Range 7.8 - 500 pg/mL
- Sensitivity Typical limit of detection (LOD) for rhGDNF is <5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
- Regulatory Status For research use only.
Product Info
-
Product Description
The Biosensis GDNF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and guinea pig serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.
This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards.
This GDNF ELISA kit employs a recombinant human GDNF standard obtained from the National Institute for Biological Standards and Control (NIBSC) and is therefore designed to accurately measure the human form of GDNF. Note that the antibodies used in this kit cross-react with guinea pig, rat and mouse GDNF.
Guinea pig GDNF has been quantified in serum using the Human GDNF RapidTM ELISA kit, since guinea pig GDNF protein shares closest amino acid sequence homology with human GDNF. Protein levels can be expressed as human GDNF equivalents.
This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures. -
Related Products
Glial cell line-derived neurotrophic factor (GDNF), Mouse, Rapid™ ELISA assay
Glial cell line-derived neurotrophic factor (GDNF), Rat, Rapid™ ELISA assay
- Application(s) ELISA
- Application Details ELISA. For the quantification of Glial cell line-derived neurotrophic factor (GDNF) in Culture Supernatant, Cell Lysates, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Glial cell line-derived neurotrophic factor (GDNF)
- Specificity Human. The antibodies used in this kit detect guinea pig, mouse and rat GDNF. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
- Target Cross-Reactivity (ELISA) No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description Human recombinant GDNF with N-terminal methionine residue.
- Range 7.8 - 500 pg/mL
- Sensitivity Typical limit of detection (LOD) for rhGDNF is <5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
- Sample Type Cell Lysates, Culture Supernatant, Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plates, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C.
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 12 months from purchase.
- Alternative Names Glial cell line-derived neurotrophic factor; Astrocyte-derived trophic factor; ATF; GDNF;
- Uniprot Number P39905
- Uniprot Number/Name P39905 (GDNF_HUMAN)
- Scientific Background GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
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Specific References
Chen S et al. (2022) Newly Generated 3D Schwann-Like Cell Spheroids From Human Adipose-Derived Stem Cells Using a Modified Protocol. Cell Transplant. 31:9636897221093312. Application: Human cell culture supernatant.
March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine.
Widbiller M et al. (2019) Neurotrophic Proteins in Dentin and Their Effect on Trigeminal Sensory Neurons. J Endod. [Epub ahead of print]. Application: Human tooth homogenate.