Cadherin-5, Goat Polyclonal Antibody

Product Info

Product Name Cadherin-5, Goat Polyclonal Antibody
Product Description google Goat anti-Cadherin-5 Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Alternative Names CDH5; cadherin 5, type 2 (vascular endothelium); 7B4; CD144; 7B4 antigen; VE-cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium); cadherin-5; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin
Application(s) FC, ICC, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with VE-cadherin from Human.
Species Reactivity Bovine, Human, Pig
Immunogen Description A synthetic peptide consisting of amino acids, C-KQARAHGKSVPE
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-Cadherin-5 Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Application(s) FC, ICC, Pep-ELISA
Application Details Peptide ELISA (1:128,000), Immunocytochemistry (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Cadherin-5
Specificity Reacts with VE-cadherin from Human.
Target Host Species Human
Species Reactivity Bovine, Human, Pig
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, C-KQARAHGKSVPE
Sequence KQARAHGKSVPE
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names CDH5; cadherin 5, type 2 (vascular endothelium); 7B4; CD144; 7B4 antigen; VE-cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium); cadherin-5; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin
Uniprot Number Q59EA3
Uniprot Number/Name Q59EA3 (Q59EA3_HUMAN)
Scientific Background Cadherins are calcium-dependent cell adhesion proteins.
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Immunofluorescence analysis: of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Flow cytometry analysis: of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Citations & References