Product NameGrowth associated protein 43 (GAP-43), Chicken Polyclonal Antibody
Product DescriptiongoogleChicken anti-Growth associated protein 43 (GAP-43) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Alternative Namesneuron growth-associated protein 43; neuromodulin; nerve growth-related peptide GAP43; axonal membrane protein GAP-43; protein F1; calmodulin-binding protein P-57; neural phosphoprotein B-50; Growth Associated Protein 43; GAP43;
Application(s)ICC, WB
Antibody HostChicken
Antibody TypePolyclonal
SpecificityThe antibody reacts with a 43 kDa band by Western blot on bovine hippocampus lysate. It has also been used successfully for immunocytochemistry. Human, rat and bovine. It is expected that it will work on other mammal tissues due to amino acid sequence similarity.
Species ReactivityBovine, Human, Other Mammals (Predicted), Rat
Immunogen DescriptionC-terminal peptide 217-227 of rat and mouse GAP43, which is KEDPEADQEHA, to which an N terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein.
Product DescriptionChicken anti-Growth associated protein 43 (GAP-43) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Application(s)ICC, WB
Application DetailsWestern Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:1,000 - 1:5,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetGrowth associated protein 43 (GAP-43)
SpecificityThe antibody reacts with a 43 kDa band by Western blot on bovine hippocampus lysate. It has also been used successfully for immunocytochemistry. Human, rat and bovine. It is expected that it will work on other mammal tissues due to amino acid sequence similarity.
Target Host SpeciesRat
Species ReactivityBovine, Human, Other Mammals (Predicted), Rat
Antibody HostChicken
Antibody TypePolyclonal
Antibody IsotypeIgY
ConjugateUnconjugated
Immunogen DescriptionC-terminal peptide 217-227 of rat and mouse GAP43, which is KEDPEADQEHA, to which an N terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein.
Purity DescriptionConcentrated purified IgY
FormatLyophilized, without preservatives.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsStore lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative Namesneuron growth-associated protein 43; neuromodulin; nerve growth-related peptide GAP43; axonal membrane protein GAP-43; protein F1; calmodulin-binding protein P-57; neural phosphoprotein B-50; Growth Associated Protein 43; GAP43;
Scientific BackgroundGAP43 is very abundant protein which is found concentrated in neurons. One group discovered it as one of three proteins which becomes unregulated during the regeneration of the toad optic nerve (1). Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 were independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14 kDa. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.
A: Immunofluorescence analysis of GAP43 expression (green) in mixed neuron-glial cultures with C-1649-100. GAP43-IR is observed in axons and dendrites, with strong staining of the submembraneous cytoskeleton and vesicles. Cultures were co-labelled with R-1694-100, a rabbit polyclonal antibody to alpha-II-spectrin (red), and a DNA dye (blue). B: Western blot of cow hippocampus extract. C-1649-100 detects GAP43 at its expected molecular weight of ~43 kDa.
Left: Analysis of GAP43 expression in cortical neuron-glial cell culture from E20 rat by Immunocytochemistry. Staining was performed with chicken antibody to GAP43 (1:2,000, green) and rabbit antibody to alpha-II spectrin (R-1694-100, 1:1,000, red). Blue: DAPI nuclear stain. The GAP43 antibody labels protein expressed in the axonal membrane and synapses of neuronal cells, while the spectrin antibody stains the submembraneous cytoskeleton of axons and dendrites. Right: Western blot analysis of tissue and cell lysates using chicken antibody to GAP43 (1:5,000). [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. A single band at 43 kDa corresponds to GAP43. The GAP43 protein is only detected in lysates of neuronal origin. C6 rat glioma cells [6] do not express GAP43.
General ReferencesSkene JH, Willard M. Changes in axonally transported proteins during axon regeneration in toad retinal ganglion cells. J. Cell Biol. 89:86-95 (1981).
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