Product DescriptionGoat anti-Growth/differentiation factor 15 (GDF15) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, IHC, FC.
Application(s)FC, IHC, WB, Pep-ELISA
Application DetailsPeptide ELISA (1:2000), Western Blot (0.1-0.3 µg/mL), Immunohistochemistry (5 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetGrowth/differentiation factor 15 (GDF15)
SpecificityReacts with GDF15 from Human.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat (Predicted)
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-QKTDTGVSLQTYDD
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FormatLiquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage InstructionsUpon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundRegulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which contitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions (PubMed:28953886). On hepatocytes, inhibits growth hormone signaling (Uniprot).
A. G-1880-100 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. B. Western Blot: G-1880-100 (0.1 µg/mL) staining of Human Prostate lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence. C. Western Blot: G-1880-100 (0.1 µg/mL) staining of LNCaP (A) and (0.3 µg/mL) K562 (B) cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.
A. G-1880-100 (7 μg/mL) Immunohistochemical staining of paraffin-embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. B. Negative Control showing staining of paraffin-embedded Human Placenta, with no primary antibody.