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Growth-regulated alpha protein, Rat, ELISA assay
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Catalog Number
BEK-2123
- Product Name Growth-regulated alpha protein, Rat, ELISA assay
- Product Description The rat Growth-regulated alpha protein (CXCL1) Kit is a sandwich ELISA. The capture antibody is a polyclonal rat CXCL1 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known CXCL1 concentration are added to these wells and allowed to complex with the bound CXCL1 antibody. A biotinylated rat CXCL1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CXCL1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat CXCL1 in samples such as culture supernatant, serum and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names C-X-C motif chemokine 1; Cytokine-induced neutrophil chemoattractant 1; Platelet-derived growth factor-inducible protein KC; Cxcl1; Cinc1; Gro; Scyb1;
- Application(s) ELISA
- Specificity Rat Growth-regulated alpha protein
- Species Reactivity Rat
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A25-K96
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The rat Growth-regulated alpha protein (CXCL1) Kit is a sandwich ELISA. The capture antibody is a polyclonal rat CXCL1 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known CXCL1 concentration are added to these wells and allowed to complex with the bound CXCL1 antibody. A biotinylated rat CXCL1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CXCL1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat CXCL1 in samples such as culture supernatant, serum and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Growth-regulated alpha protein in Culture Supernatant, Serum, Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Growth-regulated alpha protein
- Specificity Rat Growth-regulated alpha protein
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Rat
- Species Reactivity Rat
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A25-K96
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1 pg/mL
- Sample Type Culture Supernatant, Plasma (EDTA), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names C-X-C motif chemokine 1; Cytokine-induced neutrophil chemoattractant 1; Platelet-derived growth factor-inducible protein KC; Cxcl1; Cinc1; Gro; Scyb1;
- Uniprot Number P14095
- Uniprot Number/Name P14095 (GROA_RAT)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
