HeLa cells stained with C-1700-100 (green) and a monoclonal antibody to High mobility Group B protein 1 (M-1702-100, red). The HSP27 antibody reveals strong cytoplasmic staining and penetrates into the actin rich ruffled margins. The blue stain reveals DNA in the nuclei of these cells.
Left: Analysis of HSP27 expression in HeLa cells by Immunocytochemistry with chicken antibody to HSP27 (1:1,000, green). Cells were co-stained with a mouse antibody to HSP60 (red). Blue: DAPI nuclear stain. The HSP27 antibody produces strong cytoplasmic staining, while the HSP60 antibody specifically labels mitochondria. Right: Western blot analysis of whole cell lysates with chicken antibody to HSP27 (1:2,000, red, lanes 2-7).  protein standard,  NIH-3T3 ,  C6,  HeLa,  HEK293,  SH-SY5Y,  COS-1. A strong, single band at 27 kDa corresponds to the HSP27 protein expressed in human cell lines. The antibody does not recognize rodent HSP27. The blot was simultaneously probed with a mouse antibody to beta-tubulin (green).