SequenceA DNA sequence encoding the complete human Hepatocyte Growth Factor protein isoform 1 (comprising the signal peptide, alpha and beta chains) was expressed in modified human 293 cells.
Purity Description>95%, as determined by SDS-PAGE and visualised by Coomassie Brilliant Blue
Purity %> 95%
FormatThe HGF formulation consists of 0-30% carbohydrate by weight. When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution InstructionsIt is recommended that 0.5 mL of sterile phosphate-buffered saline be added to the vial.
Storage Instructionslyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 2-8°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundHepatocyte growth factor (HGF) is a member of the plasminogen related growth factor family. The HGF protein is a dimer formed from an alpha and beta subunit linked by a disulfide bond. Both subunits originate from the endoproteolytic cleavage of a common precursor which is biologically inactive. HGF is expressed in Kupffer cella and sinusoidal endothelial cells in the liver and pancreas.