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Hepatocyte growth factor (HGF), Rat, ELISA assay
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Catalog Number
BEK-2337
- Product Name Hepatocyte growth factor (HGF), Rat, ELISA assay
-
Product Description
For quantitative detection of rat HGF in cell culture supernatants, serum, and citrate, or EDTA treated plasma samples only when used as directed
The rat Hepatocyte Growth Factor (HGF) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse HGF antibody pre-coated onto the 96-well strip plates provided in the kit. Standards (expression system for standard: NSO; Immunogen sequence: Q33-R495 (alpha) and V496-L728 (beta)) and test samples are added to the wells. A biotinylated detection polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of HGF present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse HGF in samples such as culture supernatant, serum, plasma (Citrate) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Range: 156 pg/mL - 10,000 pg/mL; Sensitivity <10 pg/mL - Alternative Names Scatter factor; Hepatopoeitin-A; SF;
- Application(s) ELISA
- Specificity Natural and recombinant Rat HGF There is no detectable cross-reactivity with other relevant proteins.
- Species Reactivity Rat
- Immunogen Description Recombinant standards and immunogens used in this ELISA are expressed in a mammalian expression system and consist of the following amino acid residues:Immunogen sequence: aa 33-495 (alpha) and aa 496-728 (beta).
- Range 156 pg/mL - 10,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
-
Product Description
For quantitative detection of rat HGF in cell culture supernatants, serum, and citrate, or EDTA treated plasma samples only when used as directed
The rat Hepatocyte Growth Factor (HGF) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse HGF antibody pre-coated onto the 96-well strip plates provided in the kit. Standards (expression system for standard: NSO; Immunogen sequence: Q33-R495 (alpha) and V496-L728 (beta)) and test samples are added to the wells. A biotinylated detection polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of HGF present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse HGF in samples such as culture supernatant, serum, plasma (Citrate) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Range: 156 pg/mL - 10,000 pg/mL; Sensitivity <10 pg/mL - Application(s) ELISA
- Application Details ELISA. For the quantification of Hepatocyte growth factor (HGF) in Culture Supernatant, Serum, Plasma (Citrate), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Hepatocyte growth factor (HGF)
- Specificity Natural and recombinant Rat HGF There is no detectable cross-reactivity with other relevant proteins.
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Rat
- Species Reactivity Rat
- Immunogen Description Recombinant standards and immunogens used in this ELISA are expressed in a mammalian expression system and consist of the following amino acid residues:Immunogen sequence: aa 33-495 (alpha) and aa 496-728 (beta).
- Range 156 pg/mL - 10,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Culture Supernatant, Plasma (Citrate), Plasma (EDTA), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date Unopened kit stable at 4°C for 6 months, at -20°C for 12 months.
- Alternative Names Scatter factor; Hepatopoeitin-A; SF;
- Uniprot Number P17945
- Uniprot Number/Name P17945 (HGF_RAT)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.