Heterophilic antibody blocker for BEK-2217, BEK-2221, BEK-2237 & BEK-2240 and similar ELISA assays

As low as US$37.00
Please choose unit size

Catalog Number
BL-004

    Product Info

  • Product Name Heterophilic antibody blocker for BEK-2217, BEK-2221, BEK-2237 & BEK-2240 and similar ELISA assays
  • Product Description google BL-004-500 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing sheep immunoreagents.

    Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-004-500:

    BEK-2217/2240, Human proBDNF; Application: Serum, Citrate-Plasma
    BEK-2221, Human NT3; Application: Plasma (Citrate and EDTA)
    BEK-2237, Human proBDNF; Application: Serum, EDTA-Plasma

    One vial of BL-004-500 contains 500 μg IgG which is sufficient as sample diluent additive for one 96-well plate.

    Other ELISA assays that use sheep assay antibodies may also benefit from addition of blocker BL-004-500, but require optimization of working concentration and assay validation for accurate results.
  • Application(s) ELISA
  • Species Reactivity Human
  • Purity Description Purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description BL-004-500 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing sheep immunoreagents.

    Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-004-500:

    BEK-2217/2240, Human proBDNF; Application: Serum, Citrate-Plasma
    BEK-2221, Human NT3; Application: Plasma (Citrate and EDTA)
    BEK-2237, Human proBDNF; Application: Serum, EDTA-Plasma

    One vial of BL-004-500 contains 500 μg IgG which is sufficient as sample diluent additive for one 96-well plate.

    Other ELISA assays that use sheep assay antibodies may also benefit from addition of blocker BL-004-500, but require optimization of working concentration and assay validation for accurate results.
  • Related Products Mature BDNF/proBDNF Combo (BEK-2211/2217), Human, Mouse, Rat, Rapid™ ELISA assay
    Brain-derived neurotrophic factor, pro- (proBDNF), Human, Mouse, Rat, Rapid™ ELISA assay
  • Application(s) ELISA
  • Application Details Immunoassay blocker to reduce or eliminate heterophilic antibody interference in sandwich ELISA assays for validated sample applications.
  • Target Heterophilic antibody immunoassay blocker for BEK-2217, BEK-2221, BEK-2237 & BEK-2240 and similar ELISA assays
  • Target Host Species Human
  • Species Reactivity Human
  • Purity Description Purified
  • Format Lyophilized.
  • Reconstitution Instructions 1) Reconstitute with 1 mL assay diluent buffer to achieve a blocker stock concentration of 500 µg/mL.
    2) Dilute this stock solution further with 24 mL assay diluent buffer to obtain the blocker working concentration of 20 µg/mL. Refer to RapidTM ELISA kit protocol for further details.

    The working concentration of this HA blocker needs to be optimized by the end-user for other ELISA assays.
  • Storage Instructions Store unopened vial at 2-8°C.
  • Storage Temperature 2-8°C
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 12352203
  • Regulatory Status For research use only.

    Citations & References