Product NameHeterophilic antibody blocker for BEK-2217, BEK-2221, BEK-2237 & BEK-2240 and similar ELISA assays
Product DescriptiongoogleBL-004-500 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing sheep immunoreagents.
Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-004-500:
BEK-2217/2240, Human proBDNF; Application: Serum, Citrate-Plasma BEK-2221, Human NT3; Application: Plasma (Citrate and EDTA) BEK-2237, Human proBDNF; Application: Serum, EDTA-Plasma
One vial of BL-004-500 contains 500 μg IgG which is sufficient as sample diluent additive for one 96-well plate.
Other ELISA assays that use sheep assay antibodies may also benefit from addition of blocker BL-004-500, but require optimization of working concentration and assay validation for accurate results.
Product DescriptionBL-004-500 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing sheep immunoreagents.
Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-004-500:
BEK-2217/2240, Human proBDNF; Application: Serum, Citrate-Plasma BEK-2221, Human NT3; Application: Plasma (Citrate and EDTA) BEK-2237, Human proBDNF; Application: Serum, EDTA-Plasma
One vial of BL-004-500 contains 500 μg IgG which is sufficient as sample diluent additive for one 96-well plate.
Other ELISA assays that use sheep assay antibodies may also benefit from addition of blocker BL-004-500, but require optimization of working concentration and assay validation for accurate results.
Application DetailsImmunoassay blocker to reduce or eliminate heterophilic antibody interference in sandwich ELISA assays for validated sample applications.
TargetHeterophilic antibody immunoassay blocker for BEK-2217, BEK-2221, BEK-2237 & BEK-2240 and similar ELISA assays
Target Host SpeciesHuman
Species ReactivityHuman
Purity DescriptionPurified
FormatLyophilized.
Reconstitution Instructions1) Reconstitute with 1 mL assay diluent buffer to achieve a blocker stock concentration of 500 µg/mL. 2) Dilute this stock solution further with 24 mL assay diluent buffer to obtain the blocker working concentration of 20 µg/mL. Refer to RapidTM ELISA kit protocol for further details.
The working concentration of this HA blocker needs to be optimized by the end-user for other ELISA assays.
Storage InstructionsStore unopened vial at 2-8°C.
Storage Temperature2-8°C
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).