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Heterophilic antibody blocker for BEK-2226 & BEK-2218 and similar ELISA assays
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Catalog Number
BL-003
- Product Name Heterophilic antibody blocker for BEK-2226 & BEK-2218 and similar ELISA assays
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Product Description
BL-003-1000 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing a combination of sheep and mouse immunoreagents.
Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-003-1000:
BEK-2226, Human proNGF; Application: Serum, Heparin-Plasma
BEK-2218, Human NT4/5; Application: Citrate-Plasma
One vial of BL-003-1000 contains 1000 μg IgG which is sufficient as sample diluent additive for one 96-well plate.
Other ELISA assays that use sheep and mouse assay antibodies may also benefit from addition of blocker BL-003-1000, but require optimization of working concentration and assay validation for accurate results. - Application(s) ELISA
- Species Reactivity Human
- Purity Description Purified
- Regulatory Status For research use only.
Product Info
-
Product Description
BL-003-1000 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing a combination of sheep and mouse immunoreagents.
Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-003-1000:
BEK-2226, Human proNGF; Application: Serum, Heparin-Plasma
BEK-2218, Human NT4/5; Application: Citrate-Plasma
One vial of BL-003-1000 contains 1000 μg IgG which is sufficient as sample diluent additive for one 96-well plate.
Other ELISA assays that use sheep and mouse assay antibodies may also benefit from addition of blocker BL-003-1000, but require optimization of working concentration and assay validation for accurate results. -
Related Products
Nerve growth factor, pro- (proNGF), Human, Rapid™ ELISA assay
Neurotrophin-4/5 (NT-4/5), Human, Mouse, Rat, Monkey, Rapid™ ELISA assay
- Application(s) ELISA
- Application Details Immunoassay blocker to reduce or eliminate heterophilic antibody interference in sandwich ELISA assays for validated sample applications.
- Target Heterophilic antibody immunoassay blocker for BEK-2226 & BEK-2218 and similar ELISA assays
- Target Host Species Human
- Species Reactivity Human
- Purity Description Purified
- Format Lyophilized.
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Reconstitution Instructions
1) Reconstitute with 1 mL assay diluent buffer to achieve a blocker stock concentration of 1000 µg/mL.
2) Dilute this stock buffer further with 24 mL assay diluent buffer to obtain the blocker working concentration of 40 µg/mL. Refer to RapidTM ELISA kit protocol for further details.
The working concentration of this HA blocker needs to be optimized by the end-user for other ELISA assays. - Storage Instructions Store unopened vial at 2-8°C.
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 12352203
- Regulatory Status For research use only.