Heterophilic antibody blocker for BEK-2226 & BEK-2218 and similar ELISA assays

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Catalog Number
BL-003

    Product Info

  • Product Name Heterophilic antibody blocker for BEK-2226 & BEK-2218 and similar ELISA assays
  • Product Description google BL-003-1000 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing a combination of sheep and mouse immunoreagents.

    Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-003-1000:

    BEK-2226, Human proNGF; Application: Serum, Heparin-Plasma
    BEK-2218, Human NT4/5; Application: Citrate-Plasma

    One vial of BL-003-1000 contains 1000 μg IgG which is sufficient as sample diluent additive for one 96-well plate.

    Other ELISA assays that use sheep and mouse assay antibodies may also benefit from addition of blocker BL-003-1000, but require optimization of working concentration and assay validation for accurate results.
  • Application(s) ELISA
  • Species Reactivity Human
  • Purity Description Purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description BL-003-1000 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing a combination of sheep and mouse immunoreagents.

    Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-003-1000:

    BEK-2226, Human proNGF; Application: Serum, Heparin-Plasma
    BEK-2218, Human NT4/5; Application: Citrate-Plasma

    One vial of BL-003-1000 contains 1000 μg IgG which is sufficient as sample diluent additive for one 96-well plate.

    Other ELISA assays that use sheep and mouse assay antibodies may also benefit from addition of blocker BL-003-1000, but require optimization of working concentration and assay validation for accurate results.
  • Related Products Nerve growth factor, pro- (proNGF), Human, Rapid™ ELISA assay
    Neurotrophin-4/5 (NT-4/5), Human, Mouse, Rat, Monkey, Rapid™ ELISA assay
  • Application(s) ELISA
  • Application Details Immunoassay blocker to reduce or eliminate heterophilic antibody interference in sandwich ELISA assays for validated sample applications.
  • Target Heterophilic antibody immunoassay blocker for BEK-2226 & BEK-2218 and similar ELISA assays
  • Target Host Species Human
  • Species Reactivity Human
  • Purity Description Purified
  • Format Lyophilized.
  • Reconstitution Instructions 1) Reconstitute with 1 mL assay diluent buffer to achieve a blocker stock concentration of 1000 µg/mL.
    2) Dilute this stock buffer further with 24 mL assay diluent buffer to obtain the blocker working concentration of 40 µg/mL. Refer to RapidTM ELISA kit protocol for further details.

    The working concentration of this HA blocker needs to be optimized by the end-user for other ELISA assays.
  • Storage Instructions Store unopened vial at 2-8°C.
  • Storage Temperature 2-8°C
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 12352203
  • Regulatory Status For research use only.

    Citations & References