Product DescriptionHuman IL1RA ELISA Kit (DIY antibody pairs and standards for assay development). Quantitate Human IL1RN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2 pg/ml.
Application DetailsELISA. For the quantification of Human IL1RA in Cell Lysates, Culture Supernatant, Serum and Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
SpecificityThis human IL1RA ELISA assay is reactive to IL1RA in human samples. There is no detectable cross-reactivity with other relevant proteins
Target Cross-Reactivity (ELISA)There is no detectable cross-reactivity with other relevant proteins.
Target Host SpeciesHuman
Immunogen DescriptionExpression system for standard: E.coli; Immunogen sequence: R26-E177
Range0.31 ng/mL - 20 ng/mL
Sensitivity< 2 pg/mL
Sample TypeCulture Supernatant, Human Milk, Plasma (Heparin), Saliva, Serum, Urine
Kit ComponentsThe ELISA kit box contains capture antibody, biotinylated detection antibody, protein standards, avidin-biotin-peroxidase reagent and detailed protocols.
Storage InstructionsStore at 2-8°C
Batch NumberPlease see item label.
Expiration DateThis kit may be stored for up to 6 months at 2°C to 8°C, and at -20°C for 12 months, from the date of shipment. Avoid multiple freeze-thaw cycles
Scientific BackgroundThe interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization.3 IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.
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