Product DescriptionTyrosine Kinase Receptor B (TrkB)-Fc Chimera, Human, Purified Recombinant Protein, suitable to Block..
TargetTyrosine Kinase Receptor C (TrkC)
Target Host SpeciesHuman
Antigen Produced InHEK293
SequenceA DNA sequence encoding the signal peptide and extracellular domain of human TrkC (amino acids 1-428) was fused to the Fc region of human IgG1 (amino acids 93-330). The chimeric protein was expressed in modified human 293 cells.
Purity Description>95%, as determined by SDS-PAGE and visualized by silver stain.
Purity %> 95%
FormatThe TrkC-Fc chimera consists of 15-55% carbohydrate by weight. When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution InstructionsIt is recommended that 0.5 mL of sterile phosphate-buffered saline be added to the vial.
Storage InstructionsLyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 2-8°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundTrkC is a member of the tropomyosin-related kinase receptor family. TrkC is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC is the receptor for neurotrophin-3 (NT-3). Signalling through TrkC leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. TrkC is a heavily glycosylated molecule with 13 potential N-linked glycosylation sites within the extracellular domain.
Lane 1: MW marker Lane 2: TrkC-Fc Chimera (5 µg) Lane 3: TrkC-Fc Chimera (5 µg) treated with PNGase F to remove potential N-linked glycans Lane 4: TrkC-Fc Chimera (5 µg) treated with a glycosidase cocktail to remove potential N- and O-linked glycans