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Insulin-like growth factor I (IGF-I), Rat, ELISA assay

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Catalog Number
BEK-2150
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    Product Info

  • Product Name Insulin-like growth factor I (IGF-I), Rat, ELISA assay
  • Product Description google The rat Insulin-like growth factor I (IGF-I) Kit is a sandwich ELISA. The capture antibody is a monoclonal rat IGF-I antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known IGF-I concentration are added to these wells and allowed to complex with the bound IGF-I antibody. A biotinylated rat IGF-I polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IGF-I present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat IGF-I in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Alternative Names IGFI; Igf1; IGF-I; IGF-1; IGF1; Insulin-like growth factor 1;
  • Application(s) ELISA
  • Specificity Rat Insulin-like growth factor I. The cross reactivity with IGF-2 is < 1%.
  • Species Reactivity Rat
  • Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, G49-A118
  • Range 62.5 pg/mL - 4,000 pg/mL
  • Sensitivity < 5 pg/mL
  • Regulatory Status For research use only.

    Specifications

  • Product Description The rat Insulin-like growth factor I (IGF-I) Kit is a sandwich ELISA. The capture antibody is a monoclonal rat IGF-I antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known IGF-I concentration are added to these wells and allowed to complex with the bound IGF-I antibody. A biotinylated rat IGF-I polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IGF-I present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat IGF-I in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Application(s) ELISA
  • Application Details ELISA. For the quantification of Insulin-like growth factor I (IGF-I) in Culture Supernatant, Cell Lysates, Serum, Plasma (EDTA), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
  • Target Insulin-like growth factor I (IGF-I)
  • Specificity Rat Insulin-like growth factor I. The cross reactivity with IGF-2 is < 1%.
  • Target Cross-Reactivity (ELISA) The cross reactivity with IGF-2 is < 1%.
  • Target Host Species Rat
  • Species Reactivity Rat
  • Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, G49-A118
  • Range 62.5 pg/mL - 4,000 pg/mL
  • Sensitivity < 5 pg/mL
  • Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
  • Detection Method Colorimetric
  • Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
  • Storage Instructions Store at 2-8°C
  • Storage Temperature 2-8°C
  • Batch Number Please see item label.
  • Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
  • Alternative Names IGFI; Igf1; IGF-I; IGF-1; IGF1; Insulin-like growth factor 1;
  • Uniprot Number P08025
  • Uniprot Number/Name P08025 (IGF1_RAT)
  • Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116158
  • Regulatory Status For research use only.