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Interferon lambda-1 (IFN-lambda-1), Human, ELISA assay
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Catalog Number
BEK-2373
- Product Name Interferon lambda-1 (IFN-lambda-1), Human, ELISA assay
- Product Description The Human Interferon lambda-1 (IFN-lambda-1) Kit is a sandwich ELISA. The capture antibody is a monoclonal antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IFN-lambda-1 concentration are added to these wells and allowed to complex with the bound IFN-lambda-1 antibody. A biotinylated IFN-lambda-2 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Human IFN-lambda-1 present in the samples. There is no significant cross-reactivity between IFN-lambda-1 and its analogs, however cross-reactivity may exist with untested analogs. The purpose of this kit is the in-vitro quantitative determination of Human IFN-lambda-2 in culture supernatant, serum and plasma (heparin, EDTA) and cell lysates.This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Human Interferon lambda-1 (IFN-lambda-1), Human, Interferon lambda-1, IFN, IFN-lambda-1, IFNL-1, IFNL1, Cytokine Zcyto21, Interleukin-29,IL-29, IL29, ZCYTO21
- Application(s) ELISA
- Specificity This Human IFN-lambda-1 ELISA assay is reactive to IFN-lambda-1 in human samples. There is no detectable cross-reactivity with other relevant proteins.
- Species Reactivity Human
- Immunogen Description Expression system for standard: NS0; Immunogen sequence: G20-T200
- Range 62.5 pg/mL – 4,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The Human Interferon lambda-1 (IFN-lambda-1) Kit is a sandwich ELISA. The capture antibody is a monoclonal antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IFN-lambda-1 concentration are added to these wells and allowed to complex with the bound IFN-lambda-1 antibody. A biotinylated IFN-lambda-2 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Human IFN-lambda-1 present in the samples. There is no significant cross-reactivity between IFN-lambda-1 and its analogs, however cross-reactivity may exist with untested analogs. The purpose of this kit is the in-vitro quantitative determination of Human IFN-lambda-2 in culture supernatant, serum and plasma (heparin, EDTA) and cell lysates.This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Human Interferon lambda-1 (IFN-lambda-1) in Culture Supernatant, Serum, Plasma (EDTA), Plasma (Heparin) and Cell Lysates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Interferon lambda-1 (IFN-lambda-1)
- Specificity This Human IFN-lambda-1 ELISA assay is reactive to IFN-lambda-1 in human samples. There is no detectable cross-reactivity with other relevant proteins.
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description Expression system for standard: NS0; Immunogen sequence: G20-T200
- Range 62.5 pg/mL – 4,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Human Interferon lambda-1 (IFN-lambda-1), Human, Interferon lambda-1, IFN, IFN-lambda-1, IFNL-1, IFNL1, Cytokine Zcyto21, Interleukin-29,IL-29, IL29, ZCYTO21
- Uniprot Number Q8IU54
- Uniprot Number/Name Q8IU54 IFNL1_HUMAN;
- Scientific Background Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression. Reference: uniprot.org
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
