1800 605-5127
+61 (0)8 8352 7711
Interleukin-1 beta (IL-1 beta), Mouse, ELISA assay
As low as
US$577.00
Only %1 left
Catalog Number
BEK-2151
- Product Name Interleukin-1 beta (IL-1 beta), Mouse, ELISA assay
- Product Description The mouse Interleukin 1 beta (IL-1b) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse IL-1b antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known IL-1b concentration are added to these wells and allowed to complex with the bound IL-1b antibody. A biotinylated mouse IL-1b polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1b present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-1b in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Interleukin-1b; IL-1b; IL1b;
- Application(s) ELISA
- Specificity Mouse IL-1b
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, V118-S269
- Range 12.5pg/mL - 800pg/mL
- Sensitivity < 1 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The mouse Interleukin 1 beta (IL-1b) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse IL-1b antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known IL-1b concentration are added to these wells and allowed to complex with the bound IL-1b antibody. A biotinylated mouse IL-1b polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1b present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-1b in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Interleukin-1 beta (IL-1 beta) in Culture Supernatant, Cell Lysates, Serum, Plasma (EDTA), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Interleukin-1 beta (IL-1 beta)
- Specificity Mouse IL-1b
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Mouse
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, V118-S269
- Range 12.5pg/mL - 800pg/mL
- Sensitivity < 1 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Interleukin-1b; IL-1b; IL1b;
- Uniprot Number P10749
- Uniprot Number/Name P10749 (IL1B_MOUSE)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
-
Specific References
Kunos, G, Jourdan T, Michael P, Aouadi M (2016), Glucan-Encapsulated siRNA for Treating Type 2 Diabetes Mellitus. US Patent Application 20160138028
Jourdan T, Godlewski G, Cinar R, Bertola A, Szanda G, Liu J, Tam J, Han T, Mukhopadhyay B, Skarulis MC, Ju C, Aouadi M, Czech MP, Kunos G. (2013) Activation of the Nlrp3 inflammasome in infiltrating macrophages by endocannabinoids mediates beta cell loss in type 2 diabetes. Nat. Med Sep;19(9):1132-40 2013 [E print]