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Interleukin-1 beta (IL-1 beta), Rat, ELISA assay
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Catalog Number
BEK-2309
- Product Name Interleukin-1 beta (IL-1 beta), Rat, ELISA assay
- Product Description The rat Interleukin 1 beta (IL-1b) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse IL-1b antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known IL-1b concentration are added to these wells and allowed to complex with the bound IL-1b antibody. A biotinylated IL-1b polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1b present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat IL-1b in samples such as culture supernatant, cell lysates, tissue homogenates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Interleukin-1b; IL-1b; IL1b; Interleukin-1;
- Application(s) ELISA
- Specificity Rat IL-1b
- Species Reactivity Rat
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, V117-S268
- Range 31.2 pg/mL - 2,000 pg/mL
- Sensitivity < 1 pg/mL, determined as concentration at 2 x SD of blank (n = 20).
- Regulatory Status For research use only.
Product Info
- Product Description The rat Interleukin 1 beta (IL-1b) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse IL-1b antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known IL-1b concentration are added to these wells and allowed to complex with the bound IL-1b antibody. A biotinylated IL-1b polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1b present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat IL-1b in samples such as culture supernatant, cell lysates, tissue homogenates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Interleukin-1 beta (IL-1 beta) in Culture Supernatant, Cell Lysates, Tissue Homogenates, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Interleukin-1 beta (IL-1 beta)
- Specificity Rat IL-1b
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Rat
- Species Reactivity Rat
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, V117-S268
- Range 31.2 pg/mL - 2,000 pg/mL
- Sensitivity < 1 pg/mL, determined as concentration at 2 x SD of blank (n = 20).
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum, Tissue Homogenates
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Interleukin-1b; IL-1b; IL1b; Interleukin-1;
- Uniprot Number Q5BKB0
- Uniprot Number/Name Q5BKB0 (Q5BKB0_RAT)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
- Specific References Zhang Yet al. (2021) SIRT1 and SIRT2 modulators reduce LPS-induced inflammation in HAPI microglial cells and protect SH-SY5Y neuronal cells in vitro. J Neural Transm (Vienna). 128(5):631-644 Application: Human, culture supernatant.