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Interleukin-18 (IL-18), Human, ELISA assay
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Catalog Number
BEK-2119
- Product Name Interleukin-18 (IL-18), Human, ELISA assay
- Product Description The human Interleukin 18 (IL-18) Kit is a sandwich ELISA. The capture antibody is a monoclonal human IL-18 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IL-18 concentration are added to these wells and allowed to complex with the bound IL-18 antibody. A biotinylated anti-human IL-18 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-18 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IL-18 in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Interleukin-18; IL-18; IL18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma; IGIF; IL1F4;
- Application(s) ELISA
- Specificity Human IL-18
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, Y33-D197
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human Interleukin 18 (IL-18) Kit is a sandwich ELISA. The capture antibody is a monoclonal human IL-18 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IL-18 concentration are added to these wells and allowed to complex with the bound IL-18 antibody. A biotinylated anti-human IL-18 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-18 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IL-18 in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Interleukin-18 (IL-18) in Culture Supernatant, Cell Lysates, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Interleukin-18 (IL-18)
- Specificity Human IL-18
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, Y33-D197
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Interleukin-18; IL-18; IL18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma; IGIF; IL1F4;
- Uniprot Number Q14116
- Uniprot Number/Name Q14116 (IL18_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
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Specific References
Fernandez MV et al. (2016) Ion efflux and influenza infection trigger NLRP3 inflammasome signaling in human dendritic cells. J Leukoc Biol. 99(5):723-34
Cordero MD et al. (2016) Mutation in cytochrome b gene of mitochondrial DNA in a family with fibromyalgia is associated with NLRP3-inflammasome activation. J Met Genet. 53(2):113-22
Bullón P et al. (2016) AMPK Phosphorylation Modulates Pain by Activation of NLRP3 Inflammasome. Antioxid Redox Signal. 24(2):157-170
Miklaszewska, M et al (2015) "Clinical validity of urinary interleukin 18 and interleukin 6 determinations in preterm newborns." Link Przegląd Lekarski 72:589-596; Appl: ELISA; Human Urine
Cordero MD et al (2013) NLRP3 Inflammasome is activated in Fibromyalgia: the effect of Coenzyme Q10 PubMed link Antioxid Redox Signal. July 25 2013 Epub ahead of print
Saroj Kumar Das Majumdar (2013) Evaluation of the diagnostic performance of new markers for acute kidney injury. Transworld Med. J. 1(1) 1-7
Vijayasimha M et al. (2013) Evaluation of the diagnostic performance of new markers for acute kidney injury associated with contrast administration. J Mahatma Gandhi Inst Med Sci. 18:116-21