Product DescriptionGoat anti-Intermediate conductance calcium-activated potassium channel protein 4 (KCNN4) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC, IHC.
Application(s)FC, ICC, IHC, WB, Pep-ELISA
Application DetailsPeptide ELISA (1:6,000), Western Blot (0.1-0.3 µg/mL), Immunohistochemistry (3.75 µg/mL), Immunocytochemistry (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetIntermediate conductance calcium-activated potassium channel protein 4 (KCNN4)
SpecificityReacts with KCNN4 / KCa3.1 from Human, Mouse.
Target Host SpeciesHuman
Species ReactivityDog, Human, Mouse, Rat
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-ERQAVNATGHLSD
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FormatLiquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage InstructionsUpon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesKCNN4; potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4; IK1; IKCA1; KCA4; KCa3.1; SK4; hIKCa1; hKCa4; hSK4; intermediate conductance calcium-activated potassium channel protein 1; putative erythrocyte intermediate conductance calcium-activated potassium Gardos channel
Scientific BackgroundForms a voltage-independent potassium channel that is activated by intracellular calcium (PubMed:26148990). Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells (PubMed:17157250, PubMed:18796614). Plays a role in the late stages of EGF-induced macropinocytosis (PubMed:24591580).
Western Blot:(0.1 µg/mL) staining of Human Cerebellum (A) + Peptide (B), NIH3T3 (C) and negative Control Adrenal Gland (D) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence. B. Immunofluorescence analysis: of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
A. Human Kidney Staining: (3.75 µg/mL) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. B. Human Liver Staining: (3.75 µg/mL) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
A. Flow cytometric analysis: of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight at 4°C (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. B. Human Adrenal Gland Staining: (3.75 µg/mL) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.