Application DetailsWestern Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:100 - 1:500 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityHuman. Species cross-reactivity includes rat and mouse.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionC-terminal peptide of human IBA1. The antibody has been made against the C-terminal peptide of human IBA1 coupled to keyhole limpet hemocyanin (KLH).
Purity DescriptionIgY Fraction
FormatLyophilized IgY preparation, with sodium azide.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesIBA-1; AIF1; AIF-1 G1; Allograft inflammatory factor 1; Ionized calcium binding adapter molecule 1; Protein G1
Scientific BackgroundActin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. (Ref: uniprot.org)
Left:Rat spinal cordsection stained with C-2118-50 (red) and nuclear DNA (DAPI stain, blue) by Immunohistochemistry.Microglia are visualized in a confocal Z stack as they are small cells spreading in three dimensions. Right:Western blot analysis of C-2118-50 (green, 1:1000) with a band at approx. 15kDa. Lane 1: protein standard (red); Lane 2: Rat spleen homogenate.