HeLa cells stained with C-1698-100 (red), and counterstained with our monoclonal antibody to LAMP1 (M-1652-100). The Lamin A/C antibody reveals strong nuclear lamina staining, while the LAMP1 antibody reveals strong cytoplasmic punctate staining of lysosomes and early endosomes. The blue stain reveals DNA in the nuclei of these cells. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.
Left: Analysis of lamin A/C expression in HeLa cells with chicken anti-lamin A/C (red, 1:2,000), costained with mouse anti-actin (green) by Immunocytochemistry. Blue: Hoechst nuclear DNA stain. The chicken antibody specifically labels the nuclear lamina, while the actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites. Right: Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken anti-lamin A/C (green, 1:1,000).  HeLa cytosol,  HeLa nuclear,  NIH-3T3 cytosol, and  NIH-3T3 nuclear fractions. Two strong bands at 65 kDa and 74 kDa correspond to lamin A and lamin C proteins, respectively, detected exclusively in the nuclear fractions. The same blot was simultaneously probed with mouse anti-GAPDH (M-1376-250, red). The single band at 37 kDa represents GAPDH protein